Performing A Wavelength Calibration From The Xcalibur Data System - Thermo Scientific Accela PDA Hardware Manual

Performing a Wavelength Calibration from the Xcalibur Data System

Thermo Scientific
The alignment of the spectrum on the diode array depends on the physical alignment of
various components of the optical bench. The alignment can become offset if the detector is
sharply jolted in shipping, for example. Such bumps and jars can slightly change the
wavelength of light reaching the photodiode array. Use the automated wavelength calibration
to determine wavelength accuracy and to correct for any misalignment.
To perform a wavelength calibration from the Xcalibur data system
1. Pump HPLC-grade methanol at 1 mL/min through the flowcell.
2. Turn on both lamps and wait one hour for the lamps to equilibrate (see
Lamps from the Xcalibur Data System"
3. After the detector has reached a stable temperature (approximately one hour after you
turn on the lamps), proceed with the calibration:
a. In the Accela PDA Direct Control dialog box, click the Calibration tab
The Calibration page appears (see
Figure 40. Calibration page of the Accela PDA Direct Control dialog box
4
Xcalibur Diagnostics for the PDA Detector
Calibrating the PDA Detector from the Xcalibur Data System
on page 35).
Figure 40).
Accela PDA Detector Hardware Manual
"Controlling the
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