Thermo Scientific Accela PDA Hardware Manual page 38

2
Installation
Installing the LightPipe Flowcell
18 Accela PDA Detector Hardware Manual
3. Unscrew the retaining block knob and remove the retaining block (see
Figure 14. View of the retaining block and the retaining block knob
Retaining
block knob
Retaining block
4. Remove the protective end caps from the ends of the LightPipe flowcell.
5. Connect the inlet port of the LightPipe flowcell:
a. Use the PEEK fitting included with the LightPipe Flowcell Kit to connect one end of
the red 0.005 in. ID, insulated inlet tubing to the flowcell inlet port. Slide the
protective insulating sleeve of the tubing as close as possible to the inlet port of the
LightPipe flowcell.
Note The insulating sleeve of the inlet tubing minimizes temperature
fluctuations, which cause baseline drift.
b. Connect the other end of the tubing to the LC column outlet.
6. Connect the outlet port of the LightPipe flowcell:
• If you are not connecting to an MS detector, use a PEEK fitting to connect the blue
0.010 in. ID, outlet tubing to the LightPipe flowcell outlet. Place the other end of the
outlet tubing into the waste reservoir. When you use an optional backpressure
regulator (see Table 7
and waste tubing.
• If you are connecting the LightPipe flowcell outlet to an MS detector, use the PEEK
fittings to connect one end of the red 0.005 in. ID, PEEK tubing (included in the
MS detector accessory kit) to the outlet port of the LightPipe flowcell and to connect
the other end of the tubing to the inlet port of the MS detector.
Retaining
bolt
1
on page 103), connect the outlet line to the low-pressure union
Figure 14).
Thermo Scientific