Thermo Scientific Accela PDA Hardware Manual page 10

Contents
Chapter 3
Chapter 4
Chapter 5
Chapter 6
x Accela PDA Detector Hardware Manual
Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .27
Thermo Foundation Instrument Configuration . . . . . . . . . . . . . . . . . . . . . . . . 28
ChromQuest Data System Instrument Configuration . . . . . . . . . . . . . . . . . . . . 30
Xcalibur Diagnostics for the PDA Detector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .33
Opening the Accela PDA Direct Control Dialog Box . . . . . . . . . . . . . . . . . . . . 34
Controlling the Lamps from the Xcalibur Data System . . . . . . . . . . . . . . . . . . . 35
Turning On the Lamps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Resetting the Lamp Lifetime . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
Setting the Startup Time for the Lamps. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
Monitoring Lamp Performance from the Xcalibur Data System . . . . . . . . . . . . 38
Preparing the PDA Detector to Monitor Lamp Performance. . . . . . . . . . . . . 38
Creating a Display Method to View Light Intensity . . . . . . . . . . . . . . . . . . . 38
Viewing an Intensity Scan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
Adjusting the Light Throughput with the Xcalibur Data System. . . . . . . . . . . . 42
Calibrating the PDA Detector from the Xcalibur Data System . . . . . . . . . . . . . 44
Performing a Dark Current Calibration from the Xcalibur Data System . . . . 44
Performing a Wavelength Calibration from the Xcalibur Data System . . . . . 49
Retrieving, Viewing, Printing, and Clearing the Event Log . . . . . . . . . . . . . . . . 57
Checking the Firmware Version . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
ChromQuest Diagnostics for the PDA Detector. . . . . . . . . . . . . . . . . . . . . . . . . . . .61
Accessing the Direct Controls for the Accela PDA Detector . . . . . . . . . . . . . . . 62
Controlling the Lamps from the ChromQuest Data System . . . . . . . . . . . . . . . 63
Monitoring Lamp Performance from the ChromQuest Data System . . . . . . . . 65
Viewing an Intensity Scan from the ChromQuest Data System. . . . . . . . . . . 65
Recording the Performance of the Lamps . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
Adjusting the Light Throughput with the ChromQuest Data System . . . . . . . . 69
Setting Up the Spectral and Discrete Channel Displays. . . . . . . . . . . . . . . . . 70
Adjusting the Attenuators . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
Calibrating the PDA Detector from the ChromQuest Data System . . . . . . . . . 73
Preparing the PDA Detector for Calibration . . . . . . . . . . . . . . . . . . . . . . . . . 73
Performing an Array Calibration from the ChromQuest Data System. . . . . . 73
Performing a Wavelength Calibration from the ChromQuest Data
System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
Displaying, Printing, and Clearing the Error Log . . . . . . . . . . . . . . . . . . . . . . . 80
Checking the Firmware Version . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81
Routine Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .83
Recommended Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
Cleaning the External Surfaces of the Detector . . . . . . . . . . . . . . . . . . . . . . . . . 85
Cleaning the LightPipe Flowcell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
Removing the LightPipe Flowcell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
Cleaning the LightPipe Flowcell with Organic Solvents. . . . . . . . . . . . . . . . . 87
Cleaning the LightPipe Flowcell with Nitric Acid . . . . . . . . . . . . . . . . . . . . . 88
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