Good Laboratory Practices - Thermo Scientific Accela PDA Hardware Manual

Preface

Good Laboratory Practices

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To obtain optimal performance from your LC system and to prevent personal injury or an
environment hazard, do the following:
• Keep good records.
• Read the manufacturers' Material Safety Data Sheets (MSDSs) for the chemicals being
used in your laboratory.
• Remove particulate matter from your samples before you inject them into the liquid
chromatograph.
• Use HPLC-grade solvents.
• Connect the drainage tubes from the pump, autosampler, and detector to an appropriate
waste receptacle. Dispose of solvents as specified by local regulations.
Keep Good Records
To help identify and isolate problems with either your equipment or your methodology,
Thermo Fisher Scientific recommends that you keep good records of all system conditions, for
example,% RSDs on retention times and peak areas, peak shape, and resolution. At a
minimum, keep a chromatogram of a typical sample and standard mixture, well documented
with system conditions, for future reference. Careful comparison of retention times, peak
shapes, peak sensitivity, and baseline noise can provide valuable clues to identifying and
solving future problems.
Chemical Toxicity
Although the large volume of toxic and flammable solvents used and stored in laboratories can
be quite dangerous, do not ignore the potential hazards posed by your samples. Take special
care to read and follow all precautions that ensure proper ventilation, storage, handling, and
disposal of both solvents and samples. Become familiar with the toxicity data and potential
hazards associated with all chemicals by referring to the manufacturers' Material Safety Data
Sheets.
Sample Preparation
Always consider the solubility of your sample in the solvent/mobile phase. Sample
precipitation can plug the column, tubing, or LightPipe flowcell causing flow restriction. This
obstruction can result in irreparable damage to the system. You can avoid particulate matter
by filtering the samples through 0.45 or 0.2 micron (or less) filters.
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