Thermo Scientific Accela PDA Hardware Manual page 70

4
Xcalibur Diagnostics for the PDA Detector
Calibrating the PDA Detector from the Xcalibur Data System
50 Accela PDA Detector Hardware Manual
b. In the Wavelength Calibration area, click Calibrate.
The preconditions page of the wavelength calibration wizard appears (see
Figure 41. Preconditions page
c. Read the preconditions, and determine if they have been met:
• If the preconditions have been met, click Next to proceed with the calibration.
• If the preconditions have not been met, click Cancel to close the wizard and
prepare the PDA detector for calibration.
Note You can click Cancel on any page of the Wavelength Calibration
wizard to stop the calibration process.
4. On page 2 of the wavelength calibration wizard (see
a. Select a peak set from the list.
The peak set should span the wavelengths you use under normal operating
conditions. Figure 42
detector uses this wavelength list to perform the wavelength calibration.
Note Xcalibur has four calibration files to choose from. For example, the
Holmium Oxide UV file contains five wavelengths in the UV region while the
other files use sets of wavelengths from both the UV and Visible wavelength
regions. The holmium oxide absorbance maxima are selected from a spectrum
published in "Holmium Oxide Solution Wavelength Standard from 240 to
640 nm - SRM 2034 (NIST Special Publication 260-54)."
shows the selection of the Holmium Oxide 5 peak set. The
Figure 42), do the following:
Thermo Scientific
Figure 41).