Calibrating The Pda Detector From The Chromquest Data System; Preparing The Pda Detector For Calibration; Performing An Array Calibration From The Chromquest Data System; Performing A Wavelength Calibration From The Chromquest Data - Thermo Scientific Accela PDA Hardware Manual

Calibrating the PDA Detector from the ChromQuest Data System

Preparing the PDA Detector for Calibration

Performing an Array Calibration from the ChromQuest Data System

Thermo Scientific
To calibrate the PDA detector from the ChromQuest data system, follow these procedures:
1. Preparing the PDA Detector for Calibration
2. Performing an Array Calibration from the ChromQuest Data System
3. Performing a Wavelength Calibration from the ChromQuest Data System
To prepare the Accela PDA Detector for calibration
1. Replace the LC column with a flow restrictor.
2. Set up the system to pump HPLC-grade water or HPLC-grade methanol through the
flowcell, and then start the solvent flow.
3. Turn on both lamps and wait one hour for the D2 lamp to equilibrate (see
the Lamps from the ChromQuest Data System"
4. Verify that the diode array is not saturated (see
ChromQuest Data System"
The Array calibration measures and corrects for the dark current produced by the diodes of
the photodiode array. The dark current is the small amount of background signal that is
produced by the diodes of the array even when both lamps are turned off. Typical dark current
values range from 2000 to 4000 counts.
The environmental conditions of your laboratory can cause the dark current of the diode
array to increase over time. For best results, perform an array calibration (dark current) after
any of the following events occurs:
• After 100 hours of use or monthly, whichever comes first
• Whenever a significant temperature change occurs
• After you move the detector
• After you replace the lamps
Because the dark current produced by the diodes rises as the temperature within the detector
rises, make sure that you warm up the lamps for one hour before you perform a dark current
calibration. Warming up the lamps for one hour equilibrates the detector to its normal
operating temperature.
5
ChromQuest Diagnostics for the PDA Detector
Calibrating the PDA Detector from the ChromQuest Data System
on page
"Adjusting the Light Throughput with the
on page 69).
Accela PDA Detector Hardware Manual
"Controlling
63).
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