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Table 5. Detector-related problems (Sheet 3 of 4)
Symptom
No peaks, or peaks
much smaller than
expected.
Broad, tailing peaks.
Detector does not
power up.
Detector does not go
into run upon
injection.
Flat baseline, portion
of spectrum missing.
Power LED is amber
Comm LED is amber
Thermo Scientific
Cause
Incorrect wavelength setting.
Lamp not on or defective.
Integrator input voltage does not match
detector output voltage.
Insufficient sample reaching the
detector.
Rise time is too long.
Poor connection at LightPipe flowcell
inlet.
Tripped circuit breaker at power outlet.
Blown detector fuse.
Power cord not connected.
Detector not receiving Start signal.
Saturation of photodiode array.
Ethernet connection may be loose.
Connection to the data system
computer has been lost.
Remedy
Check the wavelength setting. Make sure the
correct file is selected.
Make sure the lamp is lit. Verify the lamp
performance. See one of the following:
•
"Monitoring Lamp Performance from the
Xcalibur Data System"
•
"Monitoring Lamp Performance from the
ChromQuest Data System"
Replace the lamp if necessary.
Verify that the integrator is connected to
appropriate Analog Output connections on the
detector 's back panel. (See
"Installation."
) Check the attenuation setting on
the integrator.
Check the entire chromatographic system for
leaks. Check the sample injection volume.
Lower the rise time selection.
Check the end of the inlet tubing for a clean, flat
surface that is free of obstructions.
Resolve the problem; reset the circuit breaker.
Resolve the problem; replace the fuse (see
"Replacing the Fuses"
on
Connect the power cord.
Check the connection to the Run contacts on the
back panel of the detector.
Adjust the attenuators. See one of the following:
•
"Adjusting the Light Throughput with the
ChromQuest Data System"
•
"Adjusting the Light Throughput with the
Xcalibur Data System"
Check the Ethernet connection to the data
system computer.
Check the Ethernet connection to the data
system computer.
Accela PDA Detector Hardware Manual
7
Troubleshooting
Detector-Related Problems
on
page 38
on
page 65
Chapter 2,
page
95).
on
page 69
on
page 42
99
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