Pharmacia Biotech Multiphor II User Manual page 94

9. Multiphor II application kits and accessories
gel surface as this may start rehydration and prevent proper rehydration of
the entire gel. Sticky spots on the gel will form lead to bubble formation
during addition of rehydrating solution.
To prevent the gel from adhering to the U-framed glass plate, coat the plate
with Repel-Silane. Pour about 2 ml of Repel-Silane onto the glass and
distribute it evenly with a tissue. Leave it to dry for a few minutes and then
rinse it with distilled water. Remove water droplets by shaking or wiping
lightly with a tissue. Leave the plate to dry.
Place the U-framed glass plate on top of the dry gel and glass plate. Clamp
the cassette together using five FlexiClamps. Stand the assembled Reswelling
Cassette vertically.
Fill the syringe with approximately 20 ml of the rehydration solution and
remove air bubbles from the syringe. Connect the syringe to the tubing and
fill the cassette to the upper edge of the gel. Close the pinch cock.
If minor leakage occurs, the cassette may be leaned at a 45° angle. If major
leakage occurs (more than a few drops), remove the rehydration solution,
take the cassette apart and start again.
Before opening the cassette, open the pinch cock and allow the remaining
rehydration solution to flow out through the tubing by tilting the cassette.
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