Pharmacia Biotech Multiphor II User Manual page 48

To ensure that the current passes through the gel, all components of the
transfer sandwich are cut to the same size as the gels to be transferred.
8. Saturate the cathode electrode plate with distilled water and remove any
excess with absorbent paper. Place the cathode on top of the transfer
sandwich and connect the socket on the black cathode lead to the cathode
pin in the Multiphor II base.
9. Close the Multiphor II safety lid and connect the unit to the power
supply. It is recommended to run the transfer at a constant current of 0.8
2
mA/cm
Note: The current is calculated using the surface area (total length x width)
Note: For transfer times longer than one hour turn off the power supply,
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. A transfer time of approximately 1 hour is normal.
of the transfer sandwiches, and this calculation applies irrespective
of the number of transfer sandwiches in the stack.
remove the safety lid and carefully lift the cathode (top) electrode
without disturbing the filter papers or gel. Carefully pour on
additional transfer buffer to re-wetting the filter paper.
5. Operation
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