Pharmacia Biotech Multiphor II User Manual page 33

5. Operation
30
Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com
Position the empty electrode holder so it lies directly on the electrode wicks.
This will ensure even contact between the wicks and the gel and stop any
moisture from condensing on the gel surface.
Replace the safety lid on the unit and connect the Multiphor II unit to the
power supply. Set the power requirements, and start the experiment.
Typical power settings for agarose immunoelectrophoresis using buffer
chambers are: constant voltage 20 V/cm and current and power set to
maximum. Run time is 40-60 minutes. Bromophenol Blue is used as a
tracking dye.
Typical power settings for SDS PAGE electrophoresis using buffer
chambers.
Separation distance (cm) Voltage Current Power
(V)
8 600
16 1200
Ending the run
Turn off the power supply. Disconnect the Multiphor II unit from the
power supply. Take off the safety lid from the unit. Carefully remove the
electrode holder. Gently pull the wicks from the surface of the gel and
continue with detection techniques as required.
Warning! Always TURN OFF the power supply before opening the
safety lid. Although user safety is not endangered, arcing may damage
the contacts.
Time Temp
(mA) (W) (min) (°C)
50 30 100 15
50 30 165 15