Isoelectric Focusing Using Immobiline Dryplate - Pharmacia Biotech Multiphor II User Manual

5.6 Isoelectric
focusing
using
Immobiline
DryPlate
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Immobiline DryPlate is a dried polyacrylamide gel containing an
immobilized pH gradient.
Prior to use, the gel is rehydrated in distilled water or 1% Pharmalyte
solution to a thickness of 0.5 mm in a special reswelling cassette. Additives
such as urea and/or detergents can also be included at this stage.
Instructions are supplied with Immobiline DryPlate gels.
Immobiline DryPlate is run with electrode strips wetted in distilled water.
Recommended running conditions for one Immobiline DryPlate
pH gradient Voltage
(V)
4.0- 7.0 3500
4.2-4.9 3500
4.5-5.4 3500
5.0-6.0 3500
5.6-6.6 3500
Current Power
(mA) (W)
1 5
2 5
2 5
1 5
1 5
5. Operation
Time
(h)
20
20
20
20
20
37