Pharmacia Biotech Multiphor II User Manual page 26

Connect the socket of the cathode electrode to the pin at the front of the
unit and the anode pin to one of the sockets at the back.
Place the safety lid in position by matching the extensions on the back of the
lid with the openings on the base unit. Using the extensions at the back as a
hinge, connect the male and female banana plugs by pressing down firmly
on the front of the lid.
Connect Multiphor II to the power supply. Follow the recommended
electrical settings and running times given in the instructions supplied with
the precast gel.
* Approximate time, or until the Bromophenol Blue front reaches the anode buffer strip.
When the Bromophenol Blue front has reached the anodic buffer strip,
electrophoresis is complete and should be stopped.
Ending the run
Turn off the power supply. Disconnect the Multiphor II unit from the power
supply. Remove the safety lid from the unit. Carefully remove the electrode
holder. Gently pull the strips from the gel and continue with detection
techniques as required.
WARNING! Always TURN OFF the power supply before opening the
safety lid
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Running conditions
Voltage Current
(V) (mA)
Run 600 50
5. Operation
Power Time
(W) (min)
30 75*
23