Pharmacia Biotech Multiphor II User Manual page 79

9. Multiphor II application kits and accessories
Using the Sample Application Syringe, carefully fill the wells making sure
not to damage the gel. For immunoelectrophoretic techniques, begin the
electrophoresis as soon as possible to minimize diffusion. Diffusion
techniques can be performed in the Humidity Chamber.
7. For Grabar & Williams immunoelectrophoresis, troughs must be formed
in the gel after the first dimension electrophoresis. Using the Template,
outline the troughs with two parallel cuts through the gel with the Gel
Knife, and then remove the middle section using the projection on the
handle.
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