Table Of Contents - Pharmacia Biotech Multiphor II User Manual

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Contents
1. Introduction ..............................................................................3
2. Safety information.....................................................................7
3. Description of parts...................................................................9
3.1 Multiphor II Electrophoresis Unit..................................9
3.2 Multiphor II NovaBlot Unit for
electrophoretic transfer ...............................................13
4. Installation ..............................................................................15
4.1 Multiphor II Electrophoresis Unit................................15
4.2 Multiphor II NovaBlot Unit for
electrophoretic transfer................................................17
5. Operation................................................................................19
5.1 Electrophoresis using ExcelGel SDS
and buffer strips ..........................................................20
5.2 Electrophoresis using CleanGel and buffer strips.........25
5.3 Electrophoresis using buffer chambers.........................28
5.4 Isoelectric focusing using Ampholine PAGplate ...........31
5.5 Isoelectric focusing using CleanGel IEF .......................35
5.6 Isoelectric focusing using Immobiline DryPlate............37
5.7 2-D Electrophoresis using Immobiline DryStrip
and ExcelGel SDS gradient ..........................................38
5.8 Electrophoretic transfer ...............................................40
5.9 Stock solutions ............................................................47
5.10 Running conditions for precast gels...........................48
6. Maintenance............................................................................53
7. Technical specification.............................................................55
8. Trouble shooting .....................................................................57
9. Multiphor II application kits and accessories...........................61
9.1 SDS and Native PAGE, IEF Kit....................................61
9.2 Large Scale SDS and Native PAGE Kit ........................65
9.3 Agarose IEF Kit ...........................................................66
9.4 Immunoelectrophoresis Kit..........................................69
9.5 Preparative IEF Kit ......................................................73
9.6 Immobiline DryStrip Kit..............................................77
9.7 NovaBlot Kit ...............................................................78
9.8 UltroMould .................................................................79
9.9 Gradient Maker...........................................................83
9.10 Reswelling Cassette ...................................................85
9.11 Levelling Set ..............................................................88
9.12 Humidity Chamber ...................................................88