Operation - Pharmacia Biotech Multiphor II User Manual

5. Operation

This section, together with the information supplied with the precast gels,
gives all the necessary information to run most analytical electrophoresis
techniques using our precast gels. Running procedures for electrophoretic
transfer are also included.
For laboratory cast gels, use the running conditions recommended in
Electrophoresis in Practice, A Guide to Theory and Practice by Reiner
Westermeier.
Multiphor II contains two alternative electrode configurations.
IEF, SDS-PAGE and native PAGE are most conveniently performed using
EPH/IEF electrodes and buffer strips. There are two types of buffer strips:
ExcelGel buffer strips made of polyacrylamide and CleanGel buffer strips
made of special paper. The buffer strips are applied on the gel edges with the
electrodes on top.
Immunoelectrophoresis is run using EPH electrodes and buffer chambers.
The buffer chambers are located below the cooling plate with the electrodes
immersed in buffer solution. Paper wicks connect the buffer solution with
the gel.
This method can also be used for SDS-PAGE and native PAGE.
The optional car-mounted EPH electrodes (80-1106-62 and 80-1106-63) -
for electrophoresis using buffer chambers across the width of the cooling
plate - are moulded from polypropylene and support the platinum wire. The
anode cable (red) and cathode cable (black) carry female pin connectors for
attachment to the male pins at the front of the buffer tank.
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- EPH/IEF electrodes for use with buffer strips or electrode strips
- EPH electrodes for use with electrode wicks and buffer chambers
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ExcelGel
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Electrode wicks
5. Operation
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CleanGel
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