Pharmacia Biotech Multiphor II User Manual page 54

Electrophoresis system
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Programme for Immobiline DryStrip, pH 3-10 NL, 180 mm. All steps are
run at 10 °C.
Phase
1
2
3
4
Total
Programme for Immobiline DryStrip, pH 4-7, 110 mm. All steps are run at
10 °C.
Phase
1
2
3
4
Total
Programme for Immobiline DryStrip pH 4-7, 180 mm. All steps are run at
10 °C.
Phase
1
2
3
4
Total
* The optimal total number of Volt-hours for these pH gradients depends on the type of
sample, sample load (µg) and sample volume.
Option 2: Using a Manual Power Supply
The power supply should run at constant voltage with the parameters set as
below. All steps are run at 10 °C.
Running conditions for Immobiline DryStrip, pH 3-10, 110 mm.
Phase
1
2
Running conditions for Immobiline DryStrip, pH 3-10 L, 180 mm.
Phase
1
2
Running conditions for Immobiline DryStrip pH 3-10 NL 180 mm.
Phase
1
2
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Voltage (V)
Current (mA) Power (W)
500
1
500
1
3500
1
3500
1
Voltage (V)
Current (mA) Power (W)
300
1
300
1
3500
1
3500
1
Voltage (V)
Current (mA) Power (W)
500
1
500
1
3500
1
3500
1
Voltage (V)
Current (mA) Power (W)
300
1
1400
1
Voltage (V)
Current (mA) Power (W)
500
1
3500
1
Voltage (V)
Current (mA) Power (W)
500
1
3500
1
Time (h)
5
0.1
5
5
5
5
5
9.5
19.5
Time (h)
5
0.1
5
6
5
5
5
5.5
16.5
Time (h)
5
0.1
5
1
5
5
5
10
16
Time (h)
5
1
5
14-15
Time (h)
5
1
5
15-16
Time (h)
5
1
5
13
5. Operation
Vh
1
2500
10000
32400
44900*
Vh
1
1800
9500
19250
30550*
Vh
1
500
10000
35000
45500*
Vh
300
20000
Vh
500
55000
Vh
500
45000
51

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Multiphor ii novablot18-1018-0618-1016-85

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