Pharmacia Biotech Multiphor II User Manual page 52

CleanGel with Native Buffer Kit pH 8.9
Running conditions for CleanGel anodal electrophoresis with Native Buffer
Kit, pH 8.9.
Phase
pre-run 300
run
* Or until the Bromophenol Blue front reaches the anodic wick.
CleanGel with SDS Buffer Kit pH 8.0
Running conditions for CleanGel anodal electrophoresis with SDS Buffer
Kit, pH 8.0.
Phase
pre-run 200
run
* Or until the Bromophenol Blue front reaches the anodic wick.
CleanGel with Cathodal Native Buffer Kit, pH 5.5.
Running conditions for CleanGel cathodal electrophoresis with Native
Buffer Kit, pH 5.5.
Phase
pre-run 500
run
* Or until the Pyronin front reaches the cathodic wick.
CleanGel with Native Buffer Kit, pH 4.8.
Running conditions for CleanGel anodal electrophoresis with Native Buffer
Kit, pH 4.8.
Phase
pre-run 300
run
* Or until the Orange G front reaches the anodic wick.
Ampholine PAGplate for IEF
Running conditions for Ampholine PAGplate.
pH range
3.5–9–5
4.0–6.5
5.5–8.5
4.0–5.0
5.0–6.5
If half a gel is used, halve the current and power settings.
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Voltage Current
(V) (mA)
18
900 50
Voltage Current
(V) (mA)
70
600 100
Voltage Current
(V) (mA)
10
1200 28
Voltage Current
(V) (mA)
40
1300 40
Voltage (V)
1 500
2 000
1 600
1 400
2 000
Power Time
(W) (min)
10 10
30 60*
Power Time
(W) (min)
40 10
40 80*
Power Time
(W) (min)
10 10
28 50*
Power Time
(W) (min)
20 20
20 50*
Current (mA) Power (W)
50 30
25 25
50 25
50 30
15 20
5. Operation
Temp
°C
10
10
Temp
°C
10
10
Temp
°C
10
10
Temp
°C
10
10
Time (h) Temp °C
1.5 10
2.5 10
2.5 10
3.0 10
3.0 10
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