Pharmacia Biotech Multiphor II User Manual page 39

5. Operation
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Sample application
Use the same methods as for Ampholine PAGplate (see Section 4.4).
Running conditions
In principle, CleanGel is run in the same way as Ampholine PAGplate
except no electrode strips are used. The electrodes rest directly on the gel
surface.
Recommended running conditions for one CleanGel IEF 3-10
Phase Voltage
(V)
Prefocusing 700
Sample
entrance 500
Isoelectric
focusing 2000
Band
sharpening 2500
Note: If only half a gel is used, halve the current and power settings.
Detection Methods
Use the detection methods described for Ampholine PAGplate
(see Section 4.4).
Current Power
(mA) (W)
12 8
8 8
14 14
14 18
Time
(min)
20
20
90
10