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5. Operation
46
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10. When the transfer is complete, turn off the power supply and
disconnect NovaBlot from the power supply. Remove the safety lid and the
upper cathode electrode. Carefully disassemble the transfer sandwiches and
remove the immobilizing membranes for analysis. If necessary, save and
stain the gel to monitor the transfer efficiency.
Clean the electrodes with distilled water.
Note: Always turn off the power supply before opening the safety lid.
Although user safety is not endangered, arcing may damage the
contacts.
Detection methods
Following electrophoretic transfer, the membrane can be stored, stained or
probed immediately.
Further reading
Electrophoresis in Practice: A guide to theory and practice. Westermeier, R.,
Ed., (1993) VCH Verlagsgesellschaft mbH. Weinheim. Westermeier, R.
Beisiegel, U., Electrophoresis, 7, 1-18 (1986)
Kyhse-Andersen, J., J. Biochem. Biophys. Meth., 10, 203 (1984)
Naaby-Hansen, S., Lihme, A. O. F., Bog-Hansen, T.C., Bjerrum, O.J., in
Lectins-Biology, Biochemistry, Clinical Biochemistry, Walter de Gruyter &
Co., Berlin & New York, 241 (1985)
Handbook of immunoblotting of proteins. ed. Bjerrum, O. J. & Heegaard,
N. H. H., CRC Press, Florida, USA, Volume 1 Technical descriptions,
Volume II experimental and clinical applications.
Hancock, K. and Tsang, V.S.W., Anal. Biochem. 133, 157-162 (1983)
Towbin, H., Staehlin, T., Gordon, J., Proc. Natl. Acad. Sci. USA, 76, 4350-
4354 (1979)
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