Pharmacia Biotech Multiphor II User Manual page 70

9. Multiphor II application kits and accessories
To prevent the gel from sticking to the U-framed glass plate, coat the plate
with Repel-Silane.
Note: For this operation use gloves and a fume hood.
Pour about 2 ml of Repel-Silane onto the glass plate and distribute it evenly
with a tissue. Leave it to dry for a few minutes. Rinse the glass plate with
distilled water and remove water drops by shaking or wiping lightly with a
tissue. Leave the glass plate to dry.
When using the 1 mm thick glass plate as the gel support, simply lay it
directly on top of the 3 mm thick glass plate. If the gel is to be permanently
bound to the 1 mm thick glass plate, coat the plate with Bind-Silane, before
preparing the mould.
Note: For this operation use gloves and a fume hood.
Pour about 2 ml of diluted Bind-Silane onto the glass plate and distribute it
evenly with a tissue. Leave the glass plate to dry for a few minutes, rinse
with distilled water and leave to dry.
When using GelBond PAGfilm, pour a few ml of water on to the 3 mm thick
glass plate and lay the film over it with the hydrophilic side up (see
Instructions supplied with the film). Centre the film on the glass plate.
Beginning at one end, use the roller to apply even pressure over the film
surface in order to eliminate air bubbles and seal the film to the plate with a
minimum of water. Remove any excess water with a tissue.
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