Pharmacia Biotech Multiphor II User Manual page 82

9. Multiphor II application kits and accessories
Place IEF Electrode Strips soaked in the appropriate electrode solution at the
anodic and cathodic side, each on top of the strips already placed in the tray.
Cut off the protruding parts so that the strips fit exactly into the tray. If
desired, prefocus the gel.
To apply the sample with the Sample Applicator, press the applicator
through the gel bed at the desired position. Scrape out the gel in the
applicator and mix it with the prepared sample in a small beaker.
Pour the gel slurry with sample back into the applicator, remove the
applicator and allow the bed to equilibrate hydrostatically for a few minutes.
Place the electrode holder with the EPH/IEF electrode onto the Electrode
Strips and start the isoelectric focusing.
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When the separation is complete, take a paper print of the separated
proteins. Mark the anode on a sheet of PEGG Print Paper. Carefully roll the
paper onto the gel surface. Avoid trapping air bubbles between the paper
and gel. Allow the paper to remain in contact with the gel for 30 to 60
seconds. Remove the wet print carefully by peeling. Place the paper print, gel
side down, on a glass plate and dry immediately from above with a hot
stream of air from a hair dryer.
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