Pharmacia Biotech Multiphor II User Manual page 47

5. Operation
44
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5. Loosen the support film from FilmRemover by pressing the handle and
carefully lift the whole sandwich with the support film, immobilizing
membrane and gel. Turn it over (support film up, immobilizing membrane
down) and place it on the layer of three filter papers on the anode.
Carefully remove the support film. If air bubbles become trapped under the
gel, wet the surface of the gel with a few drops of electrode solution, and
gently push out the bubbles.
6. Immerse nine filter papers in cathode solution T. Place these filter papers
on top of the gel to complete the transfer sandwich.
– – –
Filter paper (6)
Dialysis membrane
Filter paper (cathodic 3)
Slab gel
Immobilizing membrane
+ + +
Filter paper (anodic 3)
Filter paper (6)
7. Several gels of the same type and size can be transferred simultaneously.
Two transfer sandwiches can be put on top of each other. The cellophane
dialysis membrane placed between each transfer sandwich prevents cross-
contamination between transfer sandwiches.
The maximum gel size is 200 x 250 mm. If small gels (125 x 250 mm) are
to be transferred, NovaBlot will accept up to four gels for simultaneous
transfer by assembling two transfer sandwich stacks side by side.
Transfer
sandwich