Maintenance - Pharmacia Biotech Multiphor II User Manual

6. Maintenance

6. Maintenance
A few standard measures are necessary to keep Multiphor II in full
functioning order.
After isoelectric focusing, remove the electrodes from the electrode holder
and rinse with distilled water to remove the strong acidic and basic
solutions. Do not submerge the cable containing the pin or socket. Air dry
or carefully dry with paper tissue. Check that the platinum wire is not
damaged.
After electrophoresis using the buffer chambers, remove the electrodes.
Rinse them in distilled water and air dry. Take care not to damage the
platinum electrodes.
Rinse the buffer chambers with distilled water between buffer changes and
after use. Do not immerse the socket connector. Air dry or carefully dry with
a paper towel.
Following electrophoretic transfer, remove all remaining filter papers from
the NovaBlot unit. Remove the anode and cathode plates and rinse them in
distilled water. Do not immerse the electrode leads in water. Leave to air dry.
For longer life of NovaBlot electrodes store them either by:
1. Placing 3 cm thick plastic foam between the electrodes as if for transfer
or
2. Store the electrodes on "backs" without foam sandwiched between.
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