Trouble Shooting - Pharmacia Biotech Multiphor II User Manual

Trouble shooting guide to PAGE
Symptom
No current reading
Uneven migration of
the dye front
Burning at slots or
accumulation of
water in the slots
Trouble shooting guide to IEF
Symptom
Current increases
with time
Sparking on the gel
Water droplets on gel
Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com

8. Trouble shooting

WARNING! Turn OFF the power supply before opening the lid.
Cause
Safety plug improperly inserted
in power supply outlet
Pin and socket connection from
electrode to base incomplete
Anode bridging
contact disconnected
Banana plug connection in safety
lid not completed
Electrode holder not seated properly
Poor contact between the electrodes
and electrode strips
Bad electrical contact between the gel
and the wicks and electrodes
Poor cooling
Polypeptide complexes are too big to
enter the gel and cause
electroendosmosis
Cause
Electrode strips applied incorrectly
in relation to electrode polarity
Cathode and anode
polarities reversed
Gel dried out, insufficient cooling
Excessive condensation
8. Trouble shooting
Remedy
Check the safety plug
insertion
Check the pin and socket
connections
Connect the bridging contact
Press firmly on the safety lid
Lower the electrode holder so
that the electrodes are in contact
with the electrode strips
Check that the electrodes are
clean and intact, and sit in the
centre of the isoelectric focusing
strips over the entire length
Check the contact
Check the cooling
If SDS PAGE, add DTT once
again and boil the sample
Remedy
Check the electrode polarity
and the pH of the electrode strips
Check the pin and socket
connections, the gel orientation
and the pH of the applied
electrode strips.
Check the temperature and flow
of the cooling fluid. Lower the
power
Decrease condensation by
adjusting the temperature of the
cooling fluid. Wipe the electrode
holder periodically to remove
condensation
57