Electrophoretic Transfer - Pharmacia Biotech Multiphor II User Manual

1. Introduction
4
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The following guide summarizes how you can expand and use Multiphor II
Electrophoresis Unit with application kits and accessories.
Application
SDS and Native PAGE
0.5x125x260 mm
homogeneous and gradient gel
SDS and Native PAGE
0.5x200x260 mm
homogeneous and gradient gel
IEF in polyacrylamide
0.1-0.5x125x260 mm
IEF in polyacrylamide
0.5x125x260 mm
IEF in agarose
Preparative IEF in granulated
gel 5x125x260 mm
Immunoelectrophoresis -
Grabar & Williams, Laurell
and fused rocket, crossed and
tandem crossed, double
diffusion
2-D, first dimension: Immobiline Immobiline DryStrip Kit
DryStrip
2-D, second dimension:
0.5x125x260 mm
2-D, second dimension:
0.5x200x260 mm

Electrophoretic transfer

This User Manual is comprised of the following sections:
1. ''Introduction'' includes a general description of Multiphor II System and
dedicated precast gels, a guide to the application kits and the manual
structure.
2. ''Description of parts'' describes in detail the components of Multiphor II
Electrophoresis Unit and Multiphor II NovaBlot Unit.
3. ''Installation'' contains a detailed description of how to install Multiphor
II Electrophoresis Unit and Multiphor II NovaBlot Unit.
4. ''Operation'' contains information on the operating procedure for SDS
and native polyacrylamide gel electrophoresis, isoelectric focusing, 2-D
electrophoresis and electrophoretic transfer.
Recommended Kit/Accessory
SDS and Native PAGE, IEF Kit
Gradient Maker
Large Scale SDS and Native
PAGE Kit
Gradient Maker
UltroMould
SDS and Native PAGE, IEF Kit
Agarose IEF Kit
Preparative IEF Kit
Immunoelectrophoresis Kit
Reswelling Cassette
SDS and Native PAGE, IEF Kit
Gradient Maker
Large Scale SDS and Native
PAGE Kit
Gradient Maker
NovaBlot Kit
FilmRemover
Code No.
18-1102-45
18-1013-72
18-1102-46
18-1013-72
18-1018-16
18-1102-45
18-1016-82
18-1018-05
18-1016-87
18-1004-30
18-1013-74
18-1102-45
18-1013-72
18-1102-46
18-1013-72
18-1016-86
18-1013-75