Pharmacia Biotech Multiphor II User Manual page 71

9. Multiphor II application kits and accessories
Form the mould by placing the U-framed glass plate in position and clamp
together using four FlexiClamps.
Note: Gloves must be worn to protect the user from contact with the toxic
acrylamide solution.
Draw the gel solution into a syringe or a graduated pipette. Fill the mould,
checking that air bubbles are not trapped along the rubber U-frame or
around the slots.
When casting a gradient gel, position the mould horizontally using the
Levelling Set and place the Gradient Maker as illustrated. Lay the end of the
tubing from the Gradient Maker against the 1 mm glass plate or GelBond
PAGfilm. The slot-former will otherwise disturb the flow of the solution.
To open the 1 mm glass plate mould, remove the four FlexiClamps.
Carefully insert one or two thin-bladed spatulas between the gel surface and
slot former on one of the short sides. Twist gently in order to introduce air
across the whole of the short side. Twist more firmly to slowly separate the
U-frame from the gel surface. Remove the U-frame. Carefully remove any
unpolymerized acrylamide from the edge of the gel with a paper tissue.
Separate the gel support from the thick glass plate. The gel is now ready to
use.
To open the mould including GelBond PAGfilm, remove the four
FlexiClamps and insert the spatula between the 3 mm thick glass plate and
film. Remove the glass plate and dry the back of the film. Turn the mould
upside down (with the glass plate with U-frame on top) and gently peel the
film with gel away from the glass.
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