Pharmacia Biotech Multiphor II User Manual page 81

9. Multiphor II application kits and accessories
Prepare the initial Ultrodex granulated gel slurry in Ampholine solution in a
beaker. Weigh the beaker and its contents. Mix the suspension by gently
stirring and immediately pour it into the tray. If the suspension does not
spread evenly gently tap the ends of the tray. Weigh the beaker and the
remaining of the gel slurry.
Mount a small fan about 70 cm above the tray and evaporate excess water
from the suspension with a gentle stream of air. The speed and distance of
the fan should be adjusted so that no ripples are formed on the surface of
the suspension.
Calculate the final weight of the prepared gel bed using the evaporation
limit shown on the side of the bottle of Ultrodex. Monitor the weight loss
until the calculated final weight is reached.
Place the prepared tray containing gel onto the cooling plate (cooled to
10 ° C) of Multiphor II. A film of water containing a non-ionic detergent
(e.g. 0.1% Triton X-100) is used to aid thermal conductivity.
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