Gradient Maker - Pharmacia Biotech Multiphor II User Manual

9.9 Gradient Maker

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This Gradient Maker comprises two compartments, the mixing chamber,
closest to the outlet, and the reservoir.
The Compensating Stick is used for linear gradients and corrects for the
density difference between the light and dense solutions and the volume of
the magnetic stirrer. The plunger is used to give a fixed volume to the mixing
chamber for preparing exponential gradients.
When using the Gradient Maker for the first time, or after it has been
disassembled for cleaning, check that it works properly by filling it with
water.
Unit contents - Code No. 18-1013-72
Designation
Gradient Maker with mixing chamber
and reservoir
Magnetic Stir Bar (2/pkg)
Plunger
Compensating Stick
Silicone Tubing 1.35/3.35 mm, 2 m
Pinch Cock (2/pkg)
Polyethylene Tubing, 1/2 mm, 5 m
Cut a 5 cm length of the silicone tubing, attach the pinch cock and attach
one end to the outlet of the Gradient Maker. At the other end of the tubing
attach a 10 cm length of the polyethylene tubing. With the pinch cock open,
check that the channel between the mixing chamber and reservoir, the outlet
and the tubing are free of water.
To move water to a position where it can easily be removed, create
additional air pressure in one of the chambers by covering the opening
completely with a thumb or heel of the hand and press down. Alternatively,
use the plunger to force air through the channel. Remaining water droplets
are removed by using a tissue.
Insert the magnetic stir bar into the mixing chamber.
9. Multiphor II application kits and accessories
Code No.
80-1315-58
18-1039-88
80-1108-68
80-1108-67
80-1065-91
80-1106-41
80-1065-75
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