Pharmacia Biotech Multiphor II User Manual page 87

9. Multiphor II application kits and accessories
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Place the Gel Casting Table in a fume cupboard. (It is very important to
work in a fume cupboard as acrylamide and bis are neurotoxins). Place the
clean long glass plate on the lower shelf of the Gel Casting Table so that one
end touches the white end screw. Pour a few ml of water close to the
opposite end of the plate and lay a GelBond PAGfilm over it with the
hydrophilic side up (see instructions supplied with the film). Align the film
with the edges of the glass plate. Beginning at one end of the film, use the
roller to apply an even pressure over the surface in order to eliminate air
bubbles and seal the film to the plate with a minimum amount of water.
Remove any excess water with a tissue.
Place the gel spacer of the desired thickness onto the glass plate with the
window in the spacer over GelBond PAGfilm.
Note: The thickness of each gel spacer is easily determined. A number of
holes relating to the thickness are punched at the end of the spacer, 5
holes = 0.5 mm, 4 holes = 0.4 mm etc.
Hold the spacer in place by screwing the black end screw into the
corresponding hole in the Gel Casting Table. The long glass plate should
now be held firmly between the black and whites screw at the two ends of
the Gel Casting Table.