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5. Operation
52
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Running conditions for Immobiline DryStrip, pH 4-7, 110 mm.
Phase
Voltage (V)
Current (mA) Power (W)
1
300
1
2
2200
1
Running conditions for Immobiline DryStrip, pH 4-7, 180 mm.
Phase
Voltage (V)
Current (mA) Power (W)
1
500
1
2
3000
1
Second dimension
Running conditions for ExcelGel XL SDS gradient 12-14.
Step
Voltage (V)
Current (mA) Power (W)
1
1000
20
2
1000
40
3
1000
40
Running conditions for ExcelGel SDS gradient 8-18.
Step
Voltage (V)
Current (mA) Power (W)
1
600
20
2
600
50
3
600
50
* When the Bromophenol Blue dye front has moved 4-6 mm for ExcelGel
XL SDS gradient 12-14 and 1-2 mm for ExcelGel SDS, gradient 8-18 from
Immobiline DryStrip, remove the strip and the application pieces.
**
When the front has moved a further 2 mm, move the cathodic buffer
strip forward to cover the area of removed Immobiline DryStrip by 1-2
mm. Adjust the position of the cathodic electrode.
***
When the Bromophenol Blue front has just reached the anodic buffer
strip, electrophoresis is continued for 5 min and should then be stopped.
Remove the buffer strips.
Further information about the gels and running conditions are supplied
with the products.
Time (h)
5
1
5
13.5
Time (h)
5
1
5
14.5
Time (min)
40
45*
40
5**
40
160***
Time (min)
30
25-30*
30
3-5**
30
70***
Vh
300
29700
Vh
500
43500
Temp.(° C)
15
15
15
Temp.(° C)
15
15
15
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