Running Conditions For Precast Gels - Pharmacia Biotech Multiphor II User Manual

5. Operation
5.10 Running
conditions
for precast
gels
48
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Transfer buffers using a discontinuous buffer system
R. Anode solution 1, pH 10.4
Tris 36.3 g
Methanol 200 ml
Make up to 1000 ml with distilled water.
S. Anode solution 2, pH 10.4
Tris 3.03 g
Methanol 200 ml
Make up to 1000 ml with distilled water.
T. Cathode solution, pH 7.6
6-Amino-n-hexanoic acid
Methanol
Make up to 1000 ml with distilled water
U. Transfer buffer using a continuous buffer system
Glycine 2.93 g
Tris 5.81 g
SDS 0.375 g
Methanol 200 ml
Make up to 1000 ml with distilled water.
Note: In a continuous buffer system, this solution is used for both anode
and cathode electrode solutions.
ExcelGel SDS gradient 8-18
Running conditions
Phase Voltage Current
(V) (mA)
Run 600 50
* Or until the Bromophenol Blue front reaches the anode buffer strip.
ExcelGel XL SDS gradient 12-14
Running conditions
Phase Voltage Current
(V) (mA)
Run 1000 40
* Or until the Bromophenol Blue front reaches the anode buffer strip.
ExcelGel SDS Homogeneous 7.5, 12.5 and 15
Running conditions
ExcelGel SDS Voltage
Homogeneous (V)
7.5 and 12.5 600
15 600
* Or until the Bromophenol Blue front reaches the anode buffer strip.
5.20 g
200 ml
Power Time
(W) (min)
30 75 *
Power Time
(W) (min)
40 165 *
Current Power
(mA) (W)
50 30
30 30
Temp
°C
15
Temp
°C
15
Time Temp
(min) °C
80* 15
140* 15