Agarose Ief Kit - Pharmacia Biotech Multiphor II User Manual

9. Multiphor II application kits and accessories

9.3 Agarose IEF Kit

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Gel Knife
Blades (5/pkg)
Template, 125x260 mm (10/pkg)
Staining Tray 2, 60x260x320 mm
Cellophane Sheets (50/pkg)
The 4 mm glass plate is used as a support for GelBond PAGfilm.
The mould comprising a 4 mm glass plate, GelBond PAGfilm and glass plate
with U-frame is clamped together using six FlexiClamps.
See Section 7.1 SDS- and Native PAGE, IEF Kit for information on
preparation of slot-former, glass plate treatment, gel casting etc.
This kit is used for casting a 0,5 mm agarose gel on GelBond film for IEF
with the carrier ampholytes - Ampholine or Pharmalyte.
The sample is applied by soaking IEF Sample Application Pieces in 15-20 µl
of sample solution and placing them on the gel surface. Alternatively,
smaller volumes, 2-4 µl, can be applied directly onto the gel surface with the
aid of the EPH/IEF Sample Application Foil.
Kit contents - Code No. 18-1016-82
Designation
Glass Plate, 125x260x3 mm (2/pkg)
Glass Plate, 125x260 mm, 0,5 mm parallel spacer (3/pkg)
Syringe, 20 ml
FlexiClamp (6/pkg)
IEF Electrode Strips (100/pkg)
IEF Sample Application. Pieces (200/pkg)
Filter paper - Electrophoresis Wick 104x253 mm (500/pkg) 80-1129-52
80-1106-37
80-1106-38
80-1129-55
18-1018-09
80-1129-38
Code No.
80-1106-99
80-1106-94
56-1135-01
18-1013-73
18-1004-40
80-1129-46