Pharmacia Biotech Multiphor II User Manual page 29

5. Operation
Rehydrate a CleanGel in an appropriate buffer using the GelPool,
according to instructions supplied with the CleanGel.
Pipette 1 ml kerosene onto the cooling plate. Position the gel in the middle
of the cooling plate with the stiff plastic support film facing down. Position
the sample wells at the cathodic or anodic side depending on the buffer
system used. No air bubbles should be trapped beneath the gel.
Place the electrode strips in the two compartments of the PaperPool. If a
smaller gel size is used, cut the strips to the correct size. Apply 20 ml of the
cathode buffer to the cathodic buffer strip using a pipette (use less buffer
volume for shorter strips). Follow the same procedure with the anode
buffer and the anode buffer strip.
Place the buffer strips onto the edges of the gel so that the strips overlap the
gel by 5 mm and there is a distance of 4 mm between the edge of the strip
and the sample wells. Place the anode buffer strip on the anode side and the
cathode buffer strip on cathode side.
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