Immobiline Drystrip Kit - Pharmacia Biotech Multiphor II User Manual

9.6 Immobiline
DryStrip Kit
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Using a spatula, transfer each gel section to a PEGG Elution Column. Add
just enough of a suitable elution buffer to each column to resuspend the gel,
and let the mixture settle until all of the buffer has entered the gel bed. Then,
carefully add one gel volume of buffer and continue the elution. The
volumes of the individual fractions must be equalized in order to measure
the UV or enzymatic profile of the separation after the elution.
This kit is used for running IEF with Immobiline DryStrip for the first
dimension in 2-D electrophoresis. Twelve strips can be focused
simultaneously under a protective layer of silicone oil.
The high sample capacity allows the application of up to 100 µl on each
Immobiline DryStrip. Detailed instructions for use are available in the
instruction manual provided with this kit.
Kit contents - Code No. 18-1004-30
Designation
Tray and Electrode Holder
DryStrip Aligner (4/pkg)
DryStrip Kit Electrode, cathode
DryStrip Kit Electrode, anode
Sample Cup Bar
Sample Cup (6x10/pkg)
IEF Electrode Strip (100/pkg)
IEF Sample Application. Piece (200/pkg)
Instruction Manual
Optional accessories
Designation
Repel-Silane, 500 ml
Reswelling Cassette
9. Multiphor II application kits and accessories
Code No.
18-1004-31
18-1004-34
18-1018-67
18-1018-66
18-1004-33
18-1004-35
18-1004-40
80-1129-46
18-1038-63
Code No.
17-1331-01
18-1013-74
77