Pharmacia Biotech Multiphor II User Manual page 46

3. Carefully place the filter papers onto the anode electrode. When forming
the first transfer sandwich, soak a further layer of three filter papers in
anode solution S (see Section 4.9, Stock solutions), using the same method
as above. Place them on top of the six filter papers on the anode electrode
plate, again taking care to avoid trapping air bubbles. When using a
continuous buffer system, all filter papers, cathodic and anodic are wetted in
the same solution.
Note: To obtain optimal transfer of molecules from the gel, care should be
4. Cut the gel loose from the support film using FilmRemover. Do not move
the gel, leave it on FilmRemover. Wet the immobilizing membrane in
electrode solution S and carefully place it on top of the gel on the
FilmRemover.
Note: Wear gloves to avoid contamination of the membrane.
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taken to avoid trapping air bubbles at all stages of the assembly of
the transfer sandwiches.
5. Operation
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