Pharmacia Biotech Multiphor II User Manual page 83

9. Multiphor II application kits and accessories
Stain the entire paper print or, if required, cut it into parallel strips for
treatment with different staining techniques (e.g. general protein stain,
zymogram stain or glycoprotein stain).
If the staining procedure is time-consuming, reapply the power to the gel to
avoid diffusion or insert the fractionating grid frame with blades
immediately after the paper print has been taken.
Locate the protein zones of interest by placing the stained print paper
directly along the tray. Press the fractionating grid frame into the gel bed.
The frame is slightly shorter than the tray which makes it possible to match
the slots for the blades with the protein zone pattern.
Select the best positions for inserting the blades. To insert a blade in the
frame, align the two extensions of a blade with the appropriate slots in the
frame. Then, lower the blade evenly. The extensions guide the narrow blade
as it cuts through the gel. Repeat this procedure for up to 20 blades.
Using a surface pH electrode, measure the pH gradient with the tray still on
the cooling plate.
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