Preparative Ief Kit - Pharmacia Biotech Multiphor II User Manual

9.5 Preparative
IEF Kit
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This kit is used for preparative IEF in a granulated gel, Ultrodex. The
separation is performed in a tray measuring 125x260x5 mm.
The maximum capacity of the tray is 150 ml of initial Ultrodex gel slurry.
Using the sample applicator, sample volumes of 3 ml for each 100 ml of
initial Ultrodex gel slurry can be applied.
The sample can be conveniently applied and separated, paper prints taken
and the gel bed fractionated. The separated proteins can then be eluted from
the granulated gel. For sample volumes larger than 5 ml, the sample can be
included in the initial gel slurry.
Kit contents - Code No. 10-1018-05
Designation
Tray, 125x260 mm, rim 5 mm (2/pkg)
IEF Electrode Strip (100/pkg)
Sample Applicator
PEGG Print Paper - Electrophoresis Wick
104x253 mm (500/pkg)
Fractionating Grid Frame
Fractionating Grid Blade (20/pkg)
PEGG Elution Column (30/pkg)
Optional accessories
Designation
Ultrodex granulated gel, 50 g
Levelling Set
Staining Tray 1, 60x150x300 mm
Clean the tray with the silicone rubber rim. Cut IEF Electrode Strips into six
10.5 cm lengths, soak the strips in a 2% Ampholine solution and place a
package of three strips at each of the short ends of the tray. Weigh the tray
and strips. Place the tray on a table levelled using the Levelling Set.
9. Multiphor II application kits and accessories
Code No.
80-1107-03
18-1004-40
80-1107-15
80-1129-52
80-1317-86
80-1108-36
80-1106-75
Code No.
80-1130-01
18-1016-88
18-1018-08
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