Pharmacia Biotech Multiphor II User Manual page 78

9. Multiphor II application kits and accessories
Leave the gels to solidify for l5 minutes at room temperature. For the best
results, store the gels in Humidity Chamber at 4 °C for one hour to allow
complete solidification. Gels may be stored in this manner for several days.
Select the appropriate Template and Gel Puncher. Slide one end of the Tygon
Tubing over the ridged section on the Gel Puncher. Using the Adapter
Connector, attach the Tygon Tubing to the vacuum tubing from a vacuum
source.
Select the appropriate Template and place it in the Plate Holder. One of the
corners of the Plate Holder is rounded to ensure that the Template is in the
correct orientation. Slide the gel underneath the Template.
Turn on the vacuum source. Place the Gel Puncher in the appropriate hole in
the Template and press it into the gel. The sharp edge of the Gel Puncher
will make a clean cut in the gel. Continue to press on the Gel Puncher until
the metal sleeve slides down, creating a vacuum which removes the gel plug.
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