Pharmacia Biotech Multiphor II User Manual page 53

5. Operation
50
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CleanGel IEF 3-10
Running conditions for one CleanGel IEF 3-10.
Phase Voltage
(V)
Prefocusing 700
Sample entry 500
Isoelectric focusing 2000
Band sharpening 2500
If half a gel is used, halve the current and power settings.
Immobiline DryPlate
Running conditions for one Immobiline DryPlate.
pH gradient Voltage
(V)
4.0- 7.0 3500
4.2-4.9 3500
4.5-5.4 3500
5.0-6.0 3500
5.6-6.6 3500
2-D electrophoresis using Immobiline DryStrip and
ExcelGel SDS gradient
First dimension
Option 1: EPS 3500 XL Power Supply, using a voltage gradient.
The parameters below may be used for up to 12 strips.
Programme for Immobiline DryStrip, pH 3-10, 110 mm. All steps are run
at 10 °C.
Phase Voltage (V) Current (mA) Power (W)
1 300 1
2 300 1
3 2000 1
4 2000 1
Total
Programme for Immobiline DryStrip, pH 3-10 L, 180 mm. All steps are run
at 10 °C.
Phase Voltage (V) Current (mA) Power (W)
1 500 1
2 500 1
3 3500 1
4 3500 1
Total
Current Power
(mA) (W)
12 8
8 8
14 14
14 18
Current Power
(mA) (W)
1 5
2 5
2 5
1 5
1 5
Time (h)
5 0.1
5 4.5
5 5
5 6.5
16
Time (h)
5 0.1
5 3
5 5
5 12.5
20.5
Time Temp
(min) °C
20 10
20 10
90 10
10 10
Time Temp
(min) °C
20 10
20 10
20 10
20 10
20 10
Vh
1
1350
5750
13000
20100*
Vh
1
1500
10000
43750
55250*