Pharmacia Biotech Multiphor II User Manual page 32

5. Operation
do this, pour a few milliliters of kerosene or light paraffin oil towards one
end of the cooling plate.
Starting with one end of the gel support, gradually lower the gel to the
horizontal position, constantly checking for trapped air bubbles. If air
becomes trapped, raise the gel just enough to release the air and then
continue to lower it onto the cooling plate. Use the template markings to
centre the gel on the cooling plate. Remove any excess solution with a
tissue. Repeat this procedure for each gel. If voltage probe measurements are
required, the gel(s) must be positioned with the direction of the current path
across the width of the cooling plate.
Prepare the electrode wicks by aligning 8-10 pieces of filter paper for each
buffer chamber. Starting at one end, slowly immerse the electrode wicks in
the buffer, using capillary action to reduce the amount of air trapped in the
paper.
When running large (195x250 mm) or medium sized gels (120x250 mm),
place the wicks so that the long edge overlaps the gel by 15 mm over the
entire length. The template markings are useful for checking that the
alignment is correct.
For running small gels (84x94 mm) on the large cooling plate, place the
wicks with the short edge overlapping the gel by 15 mm. In this case, one set
of wicks is required for each gel.
Apply the samples as required. If voltage probe measurements are not
required, remove the isoelectric focusing electrodes from the electrode
holder.
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