Pharmacia Biotech Multiphor II User Manual page 88

9. Multiphor II application kits and accessories
Making sure that the gel spacer is completely flat, attach the four holding
guides to the rails along the sides of the Gel Casting Table. The flat side
holds the spacer securely in place and guides the movement of the short glass
plate when filling the cassette. Level the assembled table using a spirit level.
This ensures that the gel solution does not collect on one side, as this may
cause leakage.
Place the short glass plate (with the Repel-Silane coated side downwards) on
the gel spacer, but not overlapping the window. Contact between the Repel-
Silane coated plate and GelBond PAGfilm should be avoided as this may
affect the film's adhesive properties. Prepare the appropriate volume of gel
solution (5, 10, 15, 20 or 25 ml) and transfer it to a beaker.
Note: Gloves must be worn to protect the user from contact with the toxic
acrylamide solution.
To obtain the correct gel thickness, apply an even and continuous pressure
to the short glass plate during the entire casting procedure. With one hand
pressed firmly on the plate, slide it so that it overlaps the window by a few
mm.
Immediately pour a few ml of gel solution onto the GelBond PAGfilm just in
front of the short glass plate. Allow the solution to completely fill the space
under the plate. Then, begin to simultaneously pour the gel solution onto the
film and slide the short glass plate over it.
If air bubbles appear, draw the plate back just far enough to release them
and then continue pouring the gel solution.
When the mould is filled and the short glass plate touches the black end
screw, place the weight on the plate to maintain pressure during
polymerization. Check that there are no air bubbles in the gel or at the
edges. Polymerization will be complete after one hour at room temperature.
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