Pharmacia Biotech Multiphor II User Manual page 45

5. Operation
1. Saturate the graphite anode plate with distilled water and remove the
excess water with absorbent paper. With the electrode lead to the front of
the instrument, fit the lower anode plate onto the buffer tank.
Connect the anode socket (red lead) to the anode pin on the right side of
the buffer tank. The transfer sandwich can now be assembled on the anode
electrode.
Note : When assembling the transfer sandwich in NovaBlot unit, always
wear gloves.
2. To ensure that the current passes only through the gel, cut the filter
papers and the immobilizing and dialysis membranes to the same size as the
gel to be transferred.
When using a discontinuous buffer system, carefully soak the first layer of
six filter papers in anode solution R (see Section 4.9 Stock solutions) by
slowly immersing the papers under the surface of the electrode solution.
Allow them to become wet by capillary action and avoid trapping air
bubbles that may interfere with the transfer.
42
Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com