Pharmacia Biotech Multiphor II User Manual page 37

5. Operation
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Place the safety lid in position by matching the extensions on the back of
the lid with the openings on the base unit. Using the extensions at the back
as a hinge, connect the male and female banana plugs by pressing down
firmly on the front of the lid.
Running conditions
Connect Multiphor II to the power supply. Set the running conditions given
in the table below. Start the isoelectric focusing by turning on the power
supply. Observe the time limits closely. If the isoelectric focusing is run too
long, the pH gradient will begin to drift towards the cathode.
Suggested running conditions for Ampholine PAGplate
pH range Voltage (V)
3.5–9–5 1 500
4.0–6.5 2 000
5.5–8.5 1 600
4.0–5.0 1 400
5.0–6.5 2 000
Note: If only half a gel is used, halve the current and power settings.
Remove the sample application pieces with forceps after approximately half
the focusing time has expired. When a sample application strip is used, let
it remain on the gel during the focusing, but remove it before placing the
gel in the fixing solution.
Ending the run
Turn off the power supply and disconnect Multiphor II from the power
supply. Remove the safety lid and electrode holder from the unit. Carefully
remove the gel and proceed with staining or preparation for the second
dimension as required.
WARNING! Always turn OFF the power supply before opening the
safety lid
Current (mA) Power (W)
50 30
25 25
50 25
50 30
15 20
Time (h)
1.5
2.5
2.5
3.0
3.0