Pharmacia Biotech Multiphor II User Manual page 36

5. Operation
Starting the IEF run
Place the electrode holder in the shallow depressions on the Multiphor II
unit.
Align the electrodes with the centre of the electrode strips by loosening the
clamping nuts and sliding the electrodes to the appropriate position.
Retighten the clamping nuts.
Lift the electrode holder slightly and reposition the supporting feet over the
deep holes. Lower carefully so that the electrodes rest on the electrode
strips. As the anodic pin and socket connectors are different for
electrophoresis using buffer chambers and isoelectric focusing, a red
bridging cable has been provided.
During isoelectric focusing, the socket on the bridging cable MUST be
attached to the pin connector.
Connect the two electrodes to the buffer tank using the spring-loaded cables
on the electrodes.
Connect the socket of the cathode electrode to the pin at the front of the
unit and the anode electrode pin to the socket at the back.
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