Page 1 Instructions for Use AutoMate 2500 Family Sample Processing System B67471B July 2018 Manufactured by Beckman Coulter, Inc. 250 S. Kraemer Blvd. Brea, CA 92821 U.S.A.
Page 2 Trademarks Beckman Coulter, the stylized logo, and the Beckman Coulter product and service marks mentioned herein are trademarks or registered trademarks of Beckman Coulter, Inc. in the United States and other countries. All other trademarks are the property of their respective owners.
Page 3: Revision History
Revision History This document applies to the latest software listed and higher versions. When a subsequent software version changes the information in this document, a new issue will be released. Following are the release dates for the original language English Instructions for Use. Localized versions have followed at different times but always correspond to the latest released version of the English manual.
Page 4 Revision History Release date Revisions May 2016 Changed maintenance schedule in order to reflect cleaning requirements for EZ Pipettor. Daily cleaning is no longer required. Daily visual inspection instead and cleaning only as required. Changes were made to the following sections: •...
Page 5 Revision History Release date Revisions July 2018 As part of AutoMate Software Version 4.1.9, changes were made to the following sections • Maintenance Schedule: Added new compressor maintenance step and clarified impacted compressors. • Oil: specified impacted compressor. • Compressor Types: listed new compressor.
Page 6 Revision History B67471B...
Page 7 Safety Notice Read all product manuals and consult with Beckman Coulter trained personnel before you operate the system. Do not perform any procedure before you carefully read all instructions. Always follow the product labels and the recommendation from the manufacturer. For more information, contact Beckman Coulter.
Page 8 Safety Notice Safety Instructions Symbol Meaning Biological risk This symbol warns of biohazardous material or risk of being contaminated. Caution This symbol indicates a potential hazard which, if not avoided, could result in minor or moderate injury. Electrical hazard This symbol denotes an area of the system that should under no circumstances be accessed as an electric shock risk exists.
Page 9 Safety Notice Safety Instructions Inspect the working environment To avoid injury, inspect your working environment for edges, corners, or protruding parts before working on the system. Avoid moving parts While the system is in operation, do not touch or go close to any moving parts. Close protective guards and covers during operation.
Page 10 Safety Notice Safety Recommendations Biological risks and hand injury when removing samples If it is necessary to remove a sample tube manually from the robot grippers, take care not to come into contact with the sample. Contamination by exceeded fill level Exceeding the maximum fill level can contaminate the device and samples.
Page 11 Customers must understand and follow all laws regarding the correct decontamination and safe disposal of electrical equipment. For Beckman Coulter products bearing this label, contact your dealer or local Beckman Coulter office for details on the take-back program that facilitates the correct collection, treatment, recovery, recycling and safe disposal of these products.
Page 12 Violation of the intended use include: • Modifications to the system installation or configuration (contact your local Beckman Coulter representative) • Use of accessories or consumables not approved by Beckman Coulter • Operation of the system by untrained staff B67471B...
Page 13 Safety Notice Owner's responsibility • Access to the system by untrained staff • Manipulation of safety devices • Disregard of the warnings and cautions in this manual • Disregard of applicable standards, guidelines and other provisions • Disregard of the service and cleaning instructions in this manual •...
Page 14 Safety Notice Machine Safety Labels Glossary of Symbols Used in AutoMate 2500 Family System Labels Symbol Standard Number And Title Of Title of Symbol And Meaning Of Symbol Standard Symbol Reference Number IEC 60878. Graphical Symbols for Warning; Biological To warn of a electrical equipment in medical Hazard, biological hazard.
Page 15 Glossary of Labels Used On AutoMate 2500 Family Systems Label Meaning Of Label Figure Callout Consult the Instructions For Use. The (19) instructions for use can be obtained by contacting your local Beckman Coulter representative obtained electronically at: www.beckmancoulter.com/ifu Caution - risk of injury from moving (1,5) parts.
Page 16 Safety Notice Machine Safety Labels Label Meaning Of Label Figure Callout AutoMate Name Rating Plate. Conveys information regarding specific AutoMate 2500 Family System. Warning; Biological Hazard. Caution - (2,10-12,17,23) risk of injury from moving parts. Consult the Instructions For Use. Normal working pressure of compressor is 6 bar / 87 psi.
Page 17 Safety Notice Machine Safety Labels Label Meaning Of Label Figure Callout Compliance label. Refer to Compliance Information for details. The numbers detailed in Figure Callout correspond with the callout numbers displayed in the figure within Position of Labels on AutoMate Systems.
Page 18 Safety Notice Machine Safety Labels Figure 2 xviii B67471B...
Page 19: Table Of Contents
Contents Revision History, iii Safety Notice, vii Introduction, xxiii CHAPTER 1: System Overview, 1-1 Introduction, 1-1 Placement of Rack on Base Frame, 1-2 Drawer Handling, 1-3 Upper Drawers, 1-3 Lower Consumable Drawers (Aliquot Module), 1-4 Sample Processing, 1-5 Tube Status Overview, 1-5 Handling Rejected Tubes, 1-7...
Page 20 Contents Distribution, 2-17 Distribution Information, 2-18 Detailed Workplace Information, 2-18 Base Frame Information, 2-20 Rack Information, 2-20 Detailed Information on Sample Tubes, 2-21 Rack and Tube Information Warning, 2-23 Actions During Distribution, 2-23 Input Drawer, 2-23 Output Drawer, 2-26 Remove Single Tube from Conveyor Belt 01, 2-28 Remove Sample Tube from Robot Grippers, 2-29...
Page 21 Contents Clean the Decapper Waste Area, 3-10 Replace Gripper Jaws of the Tube Gripper, 3-11 Robot Gripper Maintenance, 3-12 Conveyor Belt Maintenance, 3-15 Clean and Vacuum Conveyor Belts, 3-15 Tube Holders, 3-15 Inspect Guide of Conveyor Belt Base Plate, 3-19 Aliquot Module Maintenance, 3-20 Replace the Aliquotter...
Page 22 Contents Inspect the O-ring Guides for Contamination, 4-13 Recapper Troubleshooting, 4-13 Error Messages, 4-13 O-rings for the Parafilm Transport Damaged, 4-17 CHAPTER 5: Troubleshooting, 5-1 General Information, 5-1 Error Recovery During Initialization, 5-1 Complete Power Outage in the Laboratory, 5-3 Tubes Lost at Robot 1 During Operation, 5-4 System...
Page 23: Introduction
• Chemists and biologists • Trained laboratory staff All operating staff must be trained to use Beckman Coulter products on behalf of the owner. These persons must be authorized by the owner for the fulfillment of their tasks. The operator is responsible for the user maintenance procedures.
Page 24 Introduction System Options • Aliquot module to create labeled secondary tubes and detect that the sample volume is sufficient for the requested aliquots • Recapper module to reseal decapped tubes for archiving The system identifies and decaps closed, bar coded sample tubes and distributes them from an input section to an output section under the control of a Laboratory Information System (LIS).
Page 25 Introduction Definitions of Terms and Abbreviations Used in this Documentation Definitions of Terms and Abbreviations Used in this Documentation Analyzer Unit British Thermal Unit, energy unit, 1 BTU = 1,055 Joule Laboratory Information System MSDS Material Safety Data Sheets Microtiter Plates Rack ID The bar code identifying the rack, typically a four-digit number Bar code type: "NW-7 with checksum"...
Page 26 Introduction EC Declaration by the Manufacturer xxvi B67471B...
Page 27: System Overview
CHAPTER 1 System Overview Introduction Figure 1.1 AutoMate with aliquot module Sorter module Touch screen Robot 1/Robot 2 Drawers Aliquot module Robot 3 Tube drawer and printer Waste container drawer Pipette tips Recapper (optional) B67471B...
Page 28: Placement Of Rack On Base Frame
System Overview Placement of Rack on Base Frame Figure 1.2 Functional overview Input area Output area Button to open drawer Conveyor belt Get position for tubes Put position for tubes Decapping position Camera position Input rack Output rack Refer to The AutoMate 2500 Family Reference Manualfor standard and optional features. Placement of Rack on Base Frame Important Place racks on the correct position on the base frame.
Page 29: Drawer Handling
System Overview Drawer Handling Figure 1.3 Pins and indentations Figure 1.4 Wrong position and correct position Important Use tubes with the diameter and height specified for the rack. Consider label thickness on the tube when determining the tube diameter. Note Refer to The AutoMate 2500 Family Reference Manual for detailed information on base frames, racks, and trays.
Page 30: Lower Consumable Drawers (Aliquot Module)
System Overview Drawer Handling If distribution is running, the system registers the action. The LED turns orange. The system needs to complete distribution activity first. After distribution is complete, the drawer is released. The LED turns green. Lower Consumable Drawers (Aliquot Module) The drawers beneath the aliquot module provide access to consumables.
Page 31: Sample Processing
System Overview Sample Processing Sample Processing Tube Status Overview Different colors represent the tube status. The combination of fill color and border color of the dots indicates the status. Double-click to select a sample tube at its position on screen when the AutoMate is stopped and view detailed information about the tube.
Page 32 14 mm, 17 mm, or a high-density rack that can accommodate a combination of 14 mm and 17 mm tubes. To avoid errors, place tubes in the correct input rack. If you need a custom solution, contact your Beckman Coulter representative for support.
Page 33: Handling Rejected Tubes
Handling Rejected Tubes Note The AutoMate system can distribute tubes the tube inspection unit rejects, for example because the shape, cap or cap color are unknown to the system. Your Beckman Coulter representative can configure this feature. Important Changes to the LIS can be required to activate this feature. Refer your LIS provider to the AutoMate Online Specification for configuration.
Page 34: Technical Specifications
System Overview Technical Specifications Note Refer to The AutoMate 2500 Family Reference Manual for detailed information on sample processing. Technical Specifications Pump System Systems with an aliquot module (AutoMate 1250 and AutoMate 2550) have a pump system for aliquoting. Figure 1.8 Generation 1 pump (GEN1) and EZ Pipettor pump (GEN2) Aliquot volume range of the pump: 30 µl to 840 µl Note If you need to aliquot more than 840 µl, multi-aspiration or dispensation is possible.
Page 35: Pipette Tips
System Overview Technical Specifications Figure 1.9 Example: pipette filter tips (B24687) Pipette Tips Disposable tips prevent cross-contamination. Conductive pipette tips permit precise level detection. • Tip volume: 1,200 µl • Tips per rack: 140 • Racks per drawer: 3 Note Refer to The AutoMate 2500 Family Reference Manualfor detailed information on technical specifications.
Page 36 System Overview Technical Specifications Table 1.4 Tube specification for primary tubes (Continued) Characteristic Specification Material Plastic Cap types Rubber and screw caps with almost parallel sides Cap color Monochrome caps, including white multi-colored caps (speckled) Outer cap diameter 11.5 mm to 20.5 mm Cap height >...
Page 37: Run The System
Color Selection Consumable Status Sample Tube Distribution Status Operating Options Sample Tube Distribution Status Note If you have problems with the display of language-specific characters or want to use the interface in an unavailable language, contact your Beckman Coulter representative. B67471B...
Page 38: System Menus
• you need to remove a parafilm jam • your Beckman Coulter representative instructs you to When in service mode, the application background changes to orange and the system speed is decreased. To switch back to operating mode, select this menu again.
Page 39 Run the System Graphical User Interface Menu Name Description Exit Close the application. Components The Components menu lets you initialize the following single components: • Main control • Drawers • Conveyor belt 01 • Conveyor belt 02 • Host • Volume detection Select Initialize to initialize a single component.
Page 40 Run the System Graphical User Interface Menu Submenu Description Change tip Moves Robot 3 to the tip stripper, strip off the existing tip and pick up a new tip. Tube Inspection Camera active Activate or deactivate the camera. Unit After deactivating, select a specific tube type. Automatic Detection After activating, select Initialize camera...
Page 41: Operating Options
Run the System Graphical User Interface Operating Options Initialize After starting the system, select this button to initialize the system. Start Initialize After initializing the system, the button Start becomes the button. The system is in stop mode when this button is visible. Select this button to start operating the system.
Page 42: Tube Type And Cap Color Selection
Lower Consumable Drawers (Aliquot Module). Tube Type and Cap Color Selection Note Tube type and cap color should only be modified by authorized Beckman Coulter representatives. Confirm that the default setting Automatic detection is selected. Consumable Status You can see the...
Page 43: System Communication Status
Run the System Graphical User Interface System Communication Status The system indicates the status of communication between the PC and the AutoMate 2500 Family system on the left side of the screen. These indicators purely informational. Green After initialization, green indicates that initialization of the component was successful. In operating mode, green indicates that communication is idle.
Page 44: Camera Inspection Window
Run the System Preconditions Before You Start If the collision warning does not disappear after initialization, contact your Beckman Coulter representative. Camera Inspection Window Important The inspection window has to be kept minimized during operation. To prevent interface errors, do not close or exit this window. The camera inspection window always runs in the background.
Page 45: Input Drawer
Run the System Start the System Input Drawer • Confirm that there is a minimum of one sample tube on the input drawer. • In laboratories with high sample throughput, always work with a fully loaded sample tube rack to prevent interruptions in the distribution process. •...
Page 46 Run the System Start the System Turn on the AutoMate using the switch under the monitor. A beep followed by two more beeps indicate that the system is ready to be initialized. Open the door below the keyboard and switch on the PC on the right side . 2-10 B67471B...
Page 47 2. The system automatically scans for tubes on conveyor belt 1. This default setting can be deactivated by a Beckman Coulter representative. If a tube was physically available before initialization and is expected by the software but cannot be found on conveyor belt 01, a message prompts you to locate the tube and remove it.
Page 48 Run the System Start the System Note For guidance on any software messages during initialization, refer to Troubleshooting, Software Messages During Initialization. Confirm that all hardware components in the System Communication Status section of the GUI are green, which indicates successful initialization. Minimize the camera window with the checkered background by touching anywhere on the main screen other than the checkered box.
Page 49: Start The System With Sorting-Drive Connected
Run the System Start the System with Sorting-Drive Connected Load the filled input racks or tubes and close the input drawer. Press the Start icon. Note After all tubes are sorted, the robots stop moving and the input drawer opens. The system is still in operating mode.
Page 50: Startup Checklist
Run the System Startup Checklist If this message appears during startup, confirm that — Sorting-Drive Server is running. — The connection between AutoMate and Sorting-Drive is active (refer to the Sorting- Drive Programmer's Guide). Startup Checklist Turn on the system using the main power supply switch at the rear of the AutoMate. Remove all tubes from the output drawers and conveyor belt.
Page 51: Before You Start Distribution
Run the System Before You Start Distribution Confirm that all hardware components in the System Communication Status section of the GUI are green, which indicates successful initialization. Minimize the camera window. Load tubes into input racks and close the drawer. Confirm that there are enough tubes in the tube drawer, labels in the printer, and pipette tips in the tip drawer.
Page 52: Fill Up Consumables Or Empty Waste Container
Run the System Before You Start Distribution Fill Up Consumables or Empty Waste Container If you need empty the waste container or fill up the consumables during operation of the system, open the respective drawer: — Waste drawer — Tubes drawer (secondary tubes) —...
Page 53: Fill Up Disposable Pipette Tips
The drawer opens automatically (approx. 2 cm or 1 in) for you to refill. Important Only tips supplied by Beckman Coulter are validated for use. Figure 2.2 Disposable pipette filter tips Important When filling up the tips, verify that all tips are correctly placed in the rack and that no tip sticks out.
Page 54: Distribution Information
Run the System Run Distribution Note Automatic restart after reloading! After all sample tubes are distributed in operating mode, all robots stop moving. The system remains in operating mode. When you load new sample tubes onto the input drawer and close the drawer, the system resumes processing immediately.
Page 55 Run the System Run Distribution The AutoMate system uses unique workplace IDs to identify distribution destinations. Distribution destinations can be automatic or manual analyzer systems. Special sample treatment: Error (0) External destination: Hematology (410) External destination: Send outs (200) Special sample treatment: Archive rack (130) Special destination: AU 680 (310) Special destination: UniCel DxI 800 (320)
Page 56: Base Frame Information
Run the System Run Distribution The workplace name provides information about the purpose of a workplace. All alphanumeric characters are permitted. We recommend using meaningful names which anyone can understand. Examples: • Analyzers as destinations: — AU640 — AU2700 • Special sample treatment: —...
Page 57: Detailed Information On Sample Tubes
Run the System Run Distribution Detailed Information on Sample Tubes Select a workplace to open detailed information about the sample tubes located on that workplace. Figure 2.3 Sample tubes on a workplace The following Rack-Info dialog window presents information about all tubes available on that workplace.
Page 58 Run the System Run Distribution Touch any item of tube information twice, and an additional dialog window will open, presenting detailed information about the tube. The following window displays detailed information about a specific sample tube: a primary tube in this case. Sorted workplace and pipetted volume can be found here as well as the error remark.
Page 59: Rack And Tube Information Warning
Run the System Actions During Distribution Rack and Tube Information Warning The Rack Information view shows the Warning column. Warnings are indicated by: • 0: No warning • 1: Warning Select a tube with a warning to view detailed remarks about the warning in Tube Info. Actions During Distribution This section describes user actions during routine operation and interventions.
Page 60 Replace empty racks with full ones. Avoid gaps between tubes in racks. Fully loaded racks are not mandatory. Close the drawer. Note Robot 1 stops processing a rack after detecting three consecutive positions without a tube. A Beckman Coulter representative can change the number of positions if necessary. 2-24 B67471B...
Page 61 Run the System Actions During Distribution When the AutoMate is in operating mode, it immediately starts distributing tubes. Important Do not access the input drawer during the distribution process! Stop the system and reset the input drawer. Resupply Partially Filled Input Drawer Select the button on screen to open the drawer.
Page 62: Output Drawer
Run the System Actions During Distribution Output Drawer Replace Output Rack During routine operation, replace all full output racks with empty racks, after the drawer opens. After loading racks, close the drawer. The AutoMate resumes processing. The system provides two modes to handle workplaces: •...
Page 63 Run the System Actions During Distribution Figure 2.5 Workplace is full and drawer opens automatically While the drawer is open: a. Remove the tubes or full racks from the workplace. b. Add empty racks as needed. c. Select the workplace on screen. The workplace color changes to green. Figure 2.6 Workplace selected Continue routine operation.After you close the drawer, the system distributes tubes from position 1.
Page 64: Remove Single Tube From Conveyor Belt 01
Run the System Actions During Distribution If the drawer is still in use, the system does not release it. The LED below the drawer is orange. After processing all tubes, the system temporarily removes the drawer from the sorting layout. The drawer opens automatically. You can remove single tubes or complete racks.
Page 65: Remove Sample Tube From Robot Grippers
Run the System Actions During Distribution Physically remove the selected tube from the conveyor belt. Select Remove. The system prompts you to delete the tube at current position. Confirm with Yes to delete the tube at the current position. The tube disappears from the screen. Process the tube according to laboratory policy.
Page 66: Replace Pippette Tip After Error
Run the System Aliquot Module Operation If there were any problems before the tube was removed, reinitialize the robot by selecting Components>Robot 1 or Robot 2>Initialize. If you want to remove a tube from Robot 2 as well, Robot 1 must be initialized to clear the removal position for Robot 2.
Page 67 Run the System Aliquot Module Operation Locate a new secondary tube container. Place the container with tubes on a clean workplace. Confirm that the open side is facing up. Unwrap the container. Check the tube direction before loading the container. Use the sticker on the top front of the container as a guide: Position the metal tube container over the open tube container and slide it down completely.
Page 68 Run the System Aliquot Module Operation Note Make sure that the open cardboard box reaches the bottom of the metal container before turning it over. The distance between the fully inserted cardboard box and the open side of the metal container should be about 3 cm (1 in). Turn the metal container open side up: Open the package carefully and gently shake it while removing the paper.
Page 69 Run the System Aliquot Module Operation Verify that the tubes are oriented correctly. Replace the container in the drawer. Close the drawer. 2-33 B67471B...
Page 70: Screw Cap Secondary Tubes
Run the System Aliquot Module Operation While transporting and placing the container inside the drawer, avoid squeezing the container. Confirm that no tubes are in the input section (A) or tube hopper (B). The hopper is the area directly below the tube drawer. The hopper funnels tubes into the labeler gripper. The tube lift picks up tubes and places them on conveyor belt 2.
Page 71 Run the System Aliquot Module Operation Note • When an instrument has been refit, it can no longer handle standard secondary tubes. • SCSTs cannot be recapped with Parafilm by the AutoMate 2500 Recapper. • Use thermo transfer labels to label the SCSTs. Reloading SCSTs The procedure for reloading the SCSTs is the same as for standard secondary tubes, but be careful.
Page 72: Empty The Waste Container
Run the System Aliquot Module Operation Figure 2.8 Screw Cap Secondary Tubes loaded incorrectly Empty the Waste Container Caution Avoid direct contact with patient samples, disposable pipette tips, used caps, any machine components that come in contact with sample fluids, and liquid waste. Always wear gloves and other protective gear to protect yourself from infection.
Page 73: Reset The Label Counter
Run the System Aliquot Module Operation To remove the bag, lift the wire holder at the front. Secure the bag. Follow your laboratory procedure for biohazardous waste disposal. Insert a new waste bag into the container. Replace the wire holder. Close the drawer.
Page 74: Reload Pipette Tips
The alignment of Robot 3 guarantees that pipette tips are handled correctly. Your Beckman Coulter representative aligns the system during setup. Confirm that Robot 3 correctly picks up the pipette tips weekly. If the pipettor does not enter the center of the tips (you may hear the shaft knocking against the tip), the shaft may be misaligned.
Page 75: Operate Label Printer
Run the System Aliquot Module Operation Caution Risk of cross contamination if Robot 3 is misaligned and cannot pick up new tips correctly. Operate Label Printer The CAB ETS 2/300-O label printer has two operating modes: • Thermal transfer printing using transfer ribbon (ODL22049) •...
Page 76 Run the System Aliquot Module Operation Fully extend the printer drawer. Overview of Printer Components Control panel Core adapter Internal rewinder Pendulum guide Axle Printhead locking lever 2-40 B67471B...
Page 77 Contact your Beckman Coulter representative for support with printer setup, tests, or updating firmware. Icons on Graphic Display Depending on your printer configuration, any of these status icons can appear in the status line of your printer display. To configure the status line, contact your Beckman Coulter representative. 2-41 B67471B...
Page 78 Run the System Aliquot Module Operation Table 2.8 Icons on the graphic display Icon Description Time Date Digital date and time display Ribbon supply WiFi signal strength Ethernet link status Printhead temperature 2-42 B67471B...
Page 79 Run the System Aliquot Module Operation Table 2.8 Icons on the graphic display (Continued) Icon Description Debug window for abc programs An abc program controls the lower display line User memory in the clock circuit Used memory Input buffer Memory card access Printer is receiving data 2-43 B67471B...
Page 80 • Press Cancel The type or error. If this error persists, contact your Beckman Coulter representative. Power save mode If the printer is not used for awhile, it automatically switches to power save mode. The keys are not lit.
Page 81 Run the System Aliquot Module Operation • Active functions light up white in print mode (e.g. menu or feed). • Active functions light up orange in the offline menu (e.g. arrows, key 8). Table 2.10 Key functions in print mode Display State Function...
Page 82 Run the System Aliquot Module Operation Table 2.10 Key functions in print mode (Continued) Display State Function Lights Error Call help - concise information for rectifying the fault will be displayed. Table 2.11 Key functions in offline mode Menu Parameter setting Parameter choice Numeric value Return from a...
Page 83 Run the System Aliquot Module Operation Figure 2.10 Guidance of the roll in peel-off mode Core adapter Bracket Turn knob Internal rewinder Pendulum guide Axle Pinch roller Rewind assist roller Peeling edge Printhead locking lever In peel-off mode, the labels are removed after printing. The empty liner is rolled up on the internal rewinder (4).
Page 84 Run the System Aliquot Module Operation — over the two rolls of the pendulum guide (5) — under the axle (6) — through label sensor in print unit (refer to Insert a Label Strip into the Printhead — around the dispensing edge (9) —...
Page 85 Run the System Aliquot Module Operation Note Liner guided below horizontal guide of axle. Do not press down on or fold the liner. To clamp the liner to the internal rewinder: a. Hold rewinder (3) firmly and turn knob (2) clockwise until it stops. b.
Page 86 Run the System Aliquot Module Operation Figure 2.14 Remove the wound-up roll Bracket Turn knob Internal rewinder Pinch roller Rewind assist roller Peeling edge Printhead locking lever Note Dispose of the wound-up roll according to your laboratory procedure and all relevant legal requirements.
Page 87 Run the System Aliquot Module Operation Load Transfer Ribbon Figure 2.15 Feed path of the transfer ribbon Transfer ribbon feed hub Transfer ribbon roll Ribbon supply hub Printhead locking lever Before loading the transfer ribbon, clean the printhead. Turn the lever (4) counterclockwise to release the printhead. The lever is shown open in the illustration.
Page 88 Run the System Aliquot Module Operation Turn the transfer ribbon feed hub (1) counterclockwise to smooth the feed path of the transfer ribbon. Turn the lever (4) clockwise to lock the printhead. Test Label Printer Test Mode Using the Pre-dispense Key without Print Figure 2.16 Printer in test mode You can simulate a print job without printing a label or connecting to a computer.
Page 89: Detailed Rack Information
Run the System Detailed Rack Information manually removed from the pad after the first step, this step is repeated when you press the pre-dispense key again. Detailed Rack Information Error Racks Using multiple error racks for different purposes provides intelligent error rack management.
Page 90: No Order Buffer Rack
If no buffer rack is available when you start the AutoMate system, the system alerts you to configure a buffer rack. Contact your Beckman Coulter representative to configure a buffer rack. No Order Buffer Rack The No Order buffer rack is an optional feature which allows an AutoMate system to handle samples without any specified test order.
Page 91 Run the System Detailed Rack Information test order is found during reprocessing, the sample is sent to the necessary workplace. After a customer-specified maximum time period, if a test order is still not found, the AutoMate sends the sample to an error workplace. The No Order buffer rack can only be configured using the C27009 base frame and two specific racks, B43843 and B43840.
Page 92: Microtiter Plates (Mtps)
Confirm all positive results with a fresh sample. Important Only use one type of MTP per base frame for reliable operation. Your Beckman Coulter representative configures robot 3 for one type of MTP on a base frame. Using MTPs that are not configured for the base frame can cause problems.
Page 93 If host communication is interrupted (host timeout message or host communication error message), contact your network administrator. If the problem persists, contact your Beckman Coulter representative. Important Use MTPs with bar code labels in automatic mode. The rack reader detects MTP IDs when a drawer is closed.
Page 94 Run the System Detailed Rack Information The LEDs on the rack reader light up to signal that the drawer is opening. After closing the drawer, the LEDs flash to signal that the racks were identified. LED indicator Description Rack 6 OK Rack 5 OK Rack 4 OK Rack 3 OK...
Page 95 Run the System Detailed Rack Information Caution Fully extend drawers before accessing racks to prevent collision with moving components. Note Fully extend drawers before closing. A sensor detects the drawer extension. If the drawer is not completely extended, the running lights remain on. Try again. Note Do not close the drawer too fast.
Page 96 Run the System Detailed Rack Information Placing unfinished MTPs in manual mode When you place unfinished MTPs for a second run in manual mode, follow these steps: Use the same MTP IDs. Enter the IDs and start position. Return the MTP to the same position used. Caution Risk of sample mismatch.
Page 97 Run the System Detailed Rack Information • If an MTP is introduced for the second time: Is the MTP in the same position as the first run? — The LIS determines the plate position (1 to 6) from the first run. If the MTP is in a different position, the LIS rejects it.
Page 98 Run the System Detailed Rack Information Note Do not close the drawer too fast. If the drawer is closed too fast, the running lights remain on. Confirm MTPs in Manual Mode Replace a complete MTP. Before closing the drawer, select the MTP that was replaced on screen. The outline of the MTP turns yellow.
Page 99: User Maintenance
CHAPTER 3 User Maintenance Introduction to Maintenance Caution • Electrical Hazard • Power off the AutoMate system before any interventions. This general rule applies to all preventive maintenance activities. The AutoMate 2500 Family systems depend on the interaction of sensitive mechanical components and electronics to operate without failure.
Page 100: Maintenance Operation Sequence
User Maintenance Maintenance Operation Sequence Caution When lifting safety covers, lock the cover in the raised position before releasing it to avoid injury. Wear Protective Gloves Wear protective clothing and follow universal precautions as dictated by local or national regulations (CLSI GP17-A2, ISO15190 or 29CFR 1910.1030). Maintenance Operation Sequence To stop the AutoMate, press Stop Turn off the AutoMate using the switch under the monitor.
Page 101 User Maintenance Maintenance Operation Sequence Caution Always disconnect mains plug when working on electronic parts or on parts connected to the power supply. Start maintenance activities. Note When finished, remove all liquids and cleaning tools from the vicinity of the AutoMate.
Page 102: Cleaning And Decontamination
Distributors of Virkon Disinfectant Order Virkon from Beckman Coulter or contact your local distributor. Europe In Europe, the product is available under the product names RelyOn and Virkon Starter Set. United States...
Page 103: Order Numbers For Virkon Disinfectant
Three Power Tech Co.Ltd. Order Numbers for Virkon Disinfectant Use these order numbers to order Virkon from Beckman Coulter. • ODL22352: Starter set containing: — 20 Virkon tablets. Each tablet is 5 g (0.17 oz), enough for 10 L (2.6 gallons) of disinfectant solution.
Page 104: List Of Prohibited Cleaning Agents
Maintenance tasks for high volume environments are marked with a bullet (●). Consult with your supervisor to decide which tasks need to be done by Beckman Coulter service representatives. Legend: d = daily, wk = weekly, mo = monthly, 3m = every 3 months, 6m = every 6 months, t' =...
Page 105 User Maintenance Maintenance Schedule System Subsystem Description wk mo 3m 6m Complete AutoMate AutoMate Cleaning the System ● Sorter Decapper Remove tubes and caps from the decapper area ● Clean the Decapper Cap Slide - AutoMate 1200 ● and AutoMate 1250 Clean the Components of the Waste Hopper - ●...
Page 106 User Maintenance Maintenance Schedule System Subsystem Description wk mo 3m 6m Complete Clean the TIU Mirror ● Sorter Input tray Remove tubes from input tray ● Belt Clean and Vacuum the ● Conveyor Belts Check Guide in ● Conveyor Belt Base Plate for Damage Aliquoter Pump...
Page 107: Sorter Module Maintenance
User Maintenance Sorter Module Maintenance System Subsystem Description wk mo 3m 6m Complete Aliquoter 22b Jun Air 87R-4P Clean Air Intake Filter compressor ● Aliquoter Remove fallen secondary tubes and ● debris Printer Rollers Clean the Rollers ● Printhead Clean the Printhead ●...
Page 108: Clean The Components Of Waste Hopper (Automate 2500/2550)
User Maintenance Sorter Module Maintenance Clean the Components of Waste Hopper (AutoMate 2500/2550) Turn the waste hopper clockwise (1) and lift (2) to remove. Clean the components of the waste hopper with a moist absorbent tissue and a mild detergent. Wipe dry before replacing. Note AutoMate 2500 and 2550 systems have two hoppers: Clean the Decapper Waste Area...
Page 109: Replace Gripper Jaws Of The Tube Gripper
User Maintenance Sorter Module Maintenance Figure 3.1 Decapper waste area below decapper Caution Risk of hand injury caused by sharp edges. When cleaning this area, keep hands away from the edges where the metal wall meets the metal base sheet. The metal wall is flexible;...
Page 110: Robot Gripper Maintenance
User Maintenance Sorter Module Maintenance To access the other gripper jaw, open the viewing window on the back of the system and remove the other cap slide. Use a 3mm Allen wrench to loosen and remove the two screws on each bracket. Remove the gripper jaws.
Page 111: Clean The Tiu Mirror
User Maintenance Sorter Module Maintenance If the robot gripper clips are damaged or lost, replace them. Order number ODL05112 (8 pcs.) or ODL25112 (80 pcs). Check and clean the bar code reader window Check the bar code reader window for contamination. If necessary, clean the bar code reader with a moist absorbent tissue.
Page 112 User Maintenance Sorter Module Maintenance Procedure Important Turn off the power of the system before servicing. Whenever service or maintenance is necessary, it is mandatory to turn off the power of the system 30 minutes before opening the cover of the TIU. Note When working on the TIU system, always wear lint-free, cotton gloves over disposable protective gloves to protect yourself from infection, or to avoid traces of grease on the...
Page 113: Conveyor Belt Maintenance
User Maintenance Conveyor Belt Maintenance Conveyor Belt Maintenance Clean and Vacuum Conveyor Belts Always keep the conveyor belts clean! Inspect the conveyor belts for sample residue and plastic abrasion. Caution Clean the conveyor belts immediately after a tube crash or sample fluid spills. Risk of sample cross-contamination.
Page 114 Remove the screw on conveyor belt 01. Access the conveyor belt from the back of the system. Note Do not bend the metal excessively. A Beckman Coulter representative can help you remove the entire cover for thorough cleaning. Lift the cover sheet (1) and move the tube holders to position (2) to remove them.
Page 115 User Maintenance Conveyor Belt Maintenance Completely lift the cover sheet. Remove the tube holder from the removal position. Move the tube holders along the conveyor belts manually. Confirm that all tube holders move without scratching the white frame material. If a tube is in contact with the frame material, confirm that the tube holder is set in its correct position.
Page 116 User Maintenance Conveyor Belt Maintenance Reference tube holder from the side and from below Mark the reference tube holder position on the conveyor with a water resistant marker. Each tube holder has a screw on the bottom. The screw has 0.5 mm gap to the white chassis material.
Page 117: Inspect Guide Of Conveyor Belt Base Plate
User Maintenance Conveyor Belt Maintenance Soak all tube holders in a detergent solution for a minimum of 30 minutes. Scrub the tube holders using a soft brush and warm running water. After drying, replace the tube holders on the conveyor belt. Start with the reference tube holder.
Page 118: Aliquot Module Maintenance
User Maintenance Aliquot Module Maintenance Aliquot Module Maintenance Replace the Aliquotter Syringe The aliquotter syringe is located on the aliquotter robot (Robot 3). The sample syringe creates air suction and pressure to transfer sample fluid to secondary sample tubes. Over time the syringe can leak, causing inaccuracies during operation.
Page 119 User Maintenance Aliquot Module Maintenance Slowly move the syringe actuator block into its lowest position. Unscrew the top of the syringe case from the valve block. To remove the syringe from the actuator, pull the syringe towards you until the ball tip slides out of the actuator.
Page 120 User Maintenance Aliquot Module Maintenance The new syringe is delivered in two parts, the case and the plunger. Place the plunger on an absorbent tissue. Cut the tip on the spout to open the oil container. Apply a small amount of oil around the outside of the rubber plunger tip. To ensure that only a thin film of lubricant remains on the surface of the rubber tip, rub the absorbent tissue around the tip several times.
Page 121: Operation,
User Maintenance Aliquot Module Maintenance Insert the ball tip of the plunger into the actuator block. Raise the syringe so that the threads are inside the valve block. While holding the syringe in position, screw the metal thread of the case into the valve block.
Page 122: Pipettor Maintenance
User Maintenance Aliquot Module Maintenance Pipettor Maintenance The inspection and cleaning procedure depends on the type of pipettor installed. The term pipettor refers to the whole module on robot 3 used to aliquot and dispense sample liquid without the pump. The term tip carrier refers to the cylindrical metal part that picks up and carries the pipette tip.
Page 123 The cleaning agent does not corrode metal, including chrome plated steel, rubber, or plastic, and is suitable for ultrasonic baths. Order rea-sol from Beckman Coulter using this order number: • ODL20596: 1 L rea-sol...
Page 124: Inspect Tip Carrier
User Maintenance Aliquot Module Maintenance You can use other common laboratory cleaners with the same compatibility. Do not use any cleaners that contain prohibited agents! Caution Wear protective clothing and follow universal precautions as dictated by local or national regulations (CLSI GP17-A2, ISO15190 or 29CFR 1910.1030). Avoid contact with cleaning agents.
Page 125: Clean The Tip Carrier (Gen 1)
User Maintenance Aliquot Module Maintenance Carefully inspect the outside and bottom of the tip carrier for any sample residue. If the tip carrier is contaminated with sample residue, clean the tip carrier. Clean the Tip Carrier (GEN 1) You need a wide neck glass laboratory container, minimum capacity 100 ml (3.5 fl oz), maximum diameter 60 mm (2.35 in), maximum height 105 mm (4.1 in).
Page 126 User Maintenance Aliquot Module Maintenance Disconnect the left tube from the pump valve to allow the cleaning liquid to enter the tube. Important While cleaning and rinsing, do not allow any liquid to touch the clot detection module housing (marked red). 3-28 B67471B...
Page 127 User Maintenance Aliquot Module Maintenance Remove the base frames. To remove the tip stripper, turn it counterclockwise and lift it. Clean the tip stripper. Place the bottle in the bottle holder. Position the robot above the bottle. 3-29 B67471B...
Page 128 User Maintenance Aliquot Module Maintenance Take the retaining block and push the tip carrier completely down into the bottle with the cleaning solution. Figure 3.5 Retaining block (B07475) Insert and fix the retaining block between the tip carrier and pump housing to lock the tip carrier in position.
Page 129 User Maintenance Aliquot Module Maintenance Immerse the tip carrier 20 mm to 30 mm (about 1 inch) in the cleaning solution. Always leave a gap of 10 mm to 20 mm (0.5 in) between the liquid and the housing of the clot detection.
Page 130 User Maintenance Aliquot Module Maintenance Remove the tip carrier from the cleaning solution or ultrasonic bath. Rinse several times with demineralized water. To speed up the cleaning process, you can rinse several times in solvent, such as ethanol or isopropanol. Thoroughly dry the tip carrier.
Page 131: Check The Air Tightness Of The System
User Maintenance Aliquot Module Maintenance Figure 3.6 Flare end Inspect the area for contamination. Do not damage the flared end of the tubing while replacing. Replace the pipette tip base frames on the AutoMate Family system. Be sure that the entire system is dry. Press Start Wait for the sequence of three beeps.
Page 132 User Maintenance Aliquot Module Maintenance If droplets appear, the system has a leak. Inspect the connection that was opened while cleaning. Leaks must be found and repaired. If the problem persists, contact your Beckman Coulter representative. If no leak is found, resume routine operation. Clean the Tip Carrier of EZ Pipettor (GEN 2) Cleaning intervals Visually check the cleanliness of the tip carrier once a day, ideally at the end of routine...
Page 133 User Maintenance Aliquot Module Maintenance Caution Avoid direct contact with patient samples, disposable pipette tips, used caps, any machine components that come in contact with sample fluids, and liquid waste. Always wear gloves and other protective gear to protect yourself from infection. Follow all safety precautions safety...
Page 134 User Maintenance Aliquot Module Maintenance Turn the lever up to lock the pump in place. When turned up, the locking pin moves to the right. It locks the pipettor in position so that the integrated spring mechanism does not release the pipettor. Hold the tip carrier firmly.
Page 135 User Maintenance Aliquot Module Maintenance ring is missing, check if it is stuck in the retaining nut or in the centering unit. Remove Important The correct O-ring position guarantees that the pipettor can operate without failure. The O-ring should be placed in the groove on the tip carrier. Do not operate the pipettor without an O-ring.
Page 136 User Maintenance Aliquot Module Maintenance Check that the tip carrier is positioned correctly. Firmly hold the centering unit (the part just above the retaining nut) with one hand. With the other hand, try moving the tip carrier gently back and forth. The tip carrier should not move. If the tip carrier moves, remove and remount it.
Page 137 User Maintenance Aliquot Module Maintenance Replace the base frames for the pipette tips on the system. To resume processing: Turn on the AutoMate using the switch under the monitor.. Wait for the sequence of three beeps. Press Start Remove the tip carrier from the cleaning solution after 30 minutes. Dry the exterior with a lint-free absorbent tissue.
Page 138: Check Ttu Module
User Maintenance Check TTU Module Apply compressed air to the tip carrier duct to remove residue and dry the duct. When the next cleaning is due, use this tip carrier on the system while cleaning the other tip carrier. Check TTU Module Check the TTU module for contamination.
Page 139: Compressor Types
User Maintenance Air Compressor Maintenance Warning Flammable liquids can cause fires or explosions. Do not spray any flammable liquid around the compressor during operation or while it is still warm after operation. There is a high risk of fires and explosions in enclosed rooms. Compressor Types Your system may have one of the following compressor models installed.
Page 140 User Maintenance Air Compressor Maintenance Figure 3.8 Jun Air Compressor, model 6-4 without shut-off valve Figure 3.9 Planet Air L-S50-4 compressor 3-42 B67471B...
Page 141: Depressurize Compressor
User Maintenance Air Compressor Maintenance Figure 3.10 Jun-Air 87R-4P Compressor Depressurize Compressor Depressurize Compressors with or without Shut-off Valve (Jun Air models) Turn the switch from ‘I’ to ‘0’ to switch off the compressor. Figure 3.11 Jun Air Quiet Air 6-4 3-43 B67471B...
Page 142 User Maintenance Air Compressor Maintenance Figure 3.12 Jun Air 87R-4P Turn the compressor vent valve counterclockwise to depressurize the compressor. Figure 3.13 Jun Air Quiet Air 6-4 3-44 B67471B...
Page 143 User Maintenance Air Compressor Maintenance Figure 3.14 Jun Air 87R-4P Inspect the manometer to confirm that the compressor is completely depressurized. The needle points to zero or less. Depressurize Silverline Planet Air Compressor Turn the switch from ‘I’ to ‘0’ to switch off the compressor. Figure 3.15 Jun Air Quiet Air 6-4 3-45 B67471B...
Page 144: Resume Compressor Operation
User Maintenance Air Compressor Maintenance Figure 3.16 Jun Air 87R-4P To open the valve, pull the ring in the direction indicated. Inspect the manometer to confirm that the compressor is completely depressurized. The needle points to zero or less. Resume Compressor Operation Close the vent valve.
Page 145: Check Oil Level
User Maintenance Air Compressor Maintenance Check Oil Level Inspect the oil level through the porthole at the base of the Planet Air compressor. The procedure is not required for oil-free Jun-Air compressors. Figure 3.17 Oil level porthole and level indicators Add Oil Caution Before adding oil, switch off the air compressor.
Page 146: Empty The Drain Bottle
User Maintenance Air Compressor Maintenance Empty the Drain Bottle The Planet Air compressor has an automatic condensation drain system. Regularly empty the drain bottle. This procedure does not apply to the Jun-Air 87R-4P compressor. Switch off and depressurize the compressor. Unscrew the drain bottle.
Page 147: Clean Air Intake Filter
User Maintenance Air Compressor Maintenance Figure 3.18 Waste bottle of the automatic drain system Remount the drain bottle. Clean Air Intake Filter This monthly maintenance procedure applies only to the Jun-Air 87R-4P compressor. Turn off the AutoMate and the air compressor. Disconnect the air compressor from the electrical supply.
Page 148: Printer Maintenance
User Maintenance Printer Maintenance Figure 3.19 Remove the filter Clean the filter using warm, soapy water. Dry the filter before reinstalling. Install the cleaned filter. Confirm that it is installed correctly in the cover of the compressor. Reconnect the compressor to the electrical supply. Turn on the AutoMate and air compressor.
Page 149: Clean The Print Rollers
2. Remove labels and transfer ribbon from the printer. 3. Remove dirt and residue with roller cleaner and lint-free absorbent tissue. 4. If the roller appears damaged, contact your Beckman Coulter representative. 5. Clean the pinch roller, the print rollers, and the rewind assist roller.
Page 150: Clean The Label Sensor
Clean the label sensor and sensor components (2) with a brush or cotton swab soaked in pure alcohol. Reload labels and transfer ribbon. Spare Parts and Consumables Order all consumables from Beckman Coulter. Table 3.4 Spare parts and consumables Order number...
Page 151 User Maintenance Spare Parts and Consumables Table 3.4 Spare parts and consumables (Continued) Order number Part name Comment ODL25112 Gripper clip kit ODL20554 Parafilm cassette Required for recapping tubes (only if recapper option is installed). B01376 Pipette tips Required for aliquotting. Do not contain a filter.
Page 152: Service Maintenance Counter
Based on time elapsed and lab throughput a built-in maintenance counter determines when the next service maintenance is due. A message notifies you when service maintenance is due soon (Maintenance is recommended) or when it is overdue (Maintenance is required). Contact your Beckman Coulter service representative. 3-54 B67471B...
Page 153: Recapper Operation And Maintenance
CHAPTER 4 Recapper Operation and Maintenance Overview The recapper seals sample tubes with Parafilm for storage and in-house transport. Parafilm provides short-term protection against sample leakage and evaporation, so that samples can be retested. Recapper Design Parafilm is delivered on a carrier tape and placed in a Parafilm cassette. The carrier tape is wound around a separate reel.
Page 154: Recapper Control Panel
Recapper Operation and Maintenance Recapper Control Panel Figure 4.1 Recapper operation Parafilm applicator Parafilm cutting blade Parafilm supply reel Take-up reel Parafilm gripper Sample tube Note When working with the recapper, confirm that all recapped tubes are closed with Parafilm when removing racks from the system. Recapper Control Panel The recapper control panel contains two LED indicators and the operating controls.
Page 155: Leds On The Recapper Control Panel
Recapper Operation and Maintenance Recapper Control Panel Figure 4.2 Recapper control panel No function Shift No function LED keyboard active LED recapper error Operating controls LEDs on the Recapper Control Panel Symbol Description All LEDs OFF: Normal operation Green LED ON: Control panel active Red LED ON: Fault condition B67471B...
Page 156: Primary Function Of The Buttons
Recapper Operation and Maintenance Recapper Control Panel Primary Function of the Buttons Symbol Description Advance Parafilm Green LED ON: Control panel active Rewind Parafilm (rewind max. 4mm) Cut Parafilm Set to home position B67471B...
Page 157: Shift
Recapper Operation and Maintenance Deactivate Recapper Shift Press the Shift button to access the secondary function of each button. Press Shift at the same time as the function button. Secondary Function of the Buttons Symbol Description Deposit tube and open tube gripper Converge film grippers Open film grippers Turn off motors...
Page 158: Exchange The Film Cassette
Exchange the Film Cassette Revision Summary February 2017: Inserted pathway to instructional videos Important Use only the original Beckman Coulter film cassettes, order number ODL20554. Note A series of videos are installed on the AutoMate PC to help you with this task. •...
Page 159 Recapper Operation and Maintenance Exchange the Film Cassette Open the recapper hood. Cut off the paper backing: Figure 4.3 Pull on the release knob of the guide table, then fold the guide table to the lower lock position: Figure 4.4 Pull on the release knob of the film cassette and push the roll to the upper lock position: Figure 4.5 B67471B...
Page 160 Recapper Operation and Maintenance Exchange the Film Cassette Turn the cassette a short distance counterclockwise until you feel it come free. Then remove the cassette by pulling it towards the front: Figure 4.6 Turn the green clamping screw on the backing paper mandrel clockwise as far as it will Important Never rotate the black take-up manually as this will cause damage of the rotation unit!
Page 161 Recapper Operation and Maintenance Exchange the Film Cassette Figure 4.8 Break off the tab of the cassette: Figure 4.9 Unwind a length of film (approx. 80 cm) and guide the film round the guide table: Figure 4.10 Peel the film off the paper backing. Wind the paper onto the wind-up reel and push the beginning under the clamp on the wind-up reel: B67471B...
Page 162 Recapper Operation and Maintenance Exchange the Film Cassette Figure 4.11 Turn the green clamping screw counterclockwise as far as it will go to clamp the beginning of the backing paper: Figure 4.12 Important Never rotate manually the black take-up as this will cause damage of the rotation unit! Cut off the film at the mark on the guide table: 4-10...
Page 163 Recapper Operation and Maintenance Exchange the Film Cassette Figure 4.13 Pull on the release knob of the guide table, then fold the guide table back to the upper lock position: Figure 4.14 Press the button to initiate the manual film transport and hold the button depressed until the film appears at the brushes (visual check).
Page 164: Recapper Maintenance
Recapper Operation and Maintenance Recapper Maintenance Note The AutoMate system can be put into operation only when the sliding doors to the left and right of the recapper are closed. Recapper Maintenance Clean the Recapper Clean the complete recapper module weekly. Use a solution of antibacterial soap and water.
Page 165: Inspect The O-Ring Guides For Recapper Troubleshooting
Recapper Operation and Maintenance Recapper Troubleshooting Inspect the O-ring Guides for Contamination If the o-ring guides contain contamination, clean with an absorbent tissue or a cotton swab and pure alcohol. Recapper Troubleshooting Error messages help you fix common problems with the Parafilm yourself. You can also replace the O-rings if necessary.
Page 166 Recapper Operation and Maintenance Recapper Troubleshooting Cause Film is jammed near the brushes or the pressure plate. Solution Remove the Parafilm jam. Remove Parafilm Jam Revision Summary February 2017: Inserted pathway to instructional videos If film is jammed near the brushes or the pressure plate, the AutoMate stops. Compare Parafilm Found.
Page 167 Recapper Operation and Maintenance Recapper Troubleshooting Wait 30 seconds after starting maintenance mode. The interlock releases the door. Open the sliding door on the left of the recapper at the rear of the AutoMate. Open the recapper hood. Pull on the release knob of the guide table. Fold the guide table to the lower lock position.
Page 168 Recapper Operation and Maintenance Recapper Troubleshooting Press to cut the film. Remove the strip from the brushes. Close the recapper hood and the sliding door to the left side. Select OK to confirm the AutoMate software prompt. Select Yes to start initialization. Continue routine operation.
Page 169: O-Rings For The Parafilm Transport
Recapper Operation and Maintenance Recapper Troubleshooting O-rings for the Parafilm Transport Damaged If the o-rings that guide the Parafilm are damaged or cut, replace them. The recapper is equipped with a pack of three replacement parts. Unscrew the Torx 10 screw and remove the Parafilm guide. Note Do not damage the fiber-optic cables.
Page 170 Recapper Operation and Maintenance Recapper Troubleshooting Align the rectangular shaped end of the shaft along which the O-rings run with the fitting piece on the end of the driving shaft: The ends of the two shafts fit into each other. Push the Parafilm guide upwards into the highest possible position.
Page 171: Chapter 5: Troubleshooting
Note Message alerts in the system software contain unique IDs. Refer to the title bar of the message dialog. When contacting Beckman Coulter, provide this ID for support. Caution If sample fluid is spilled and contaminates the machine, remove all racks and tubes from the system.
Page 172 Troubleshooting General Information Error: Robot 1 (3022) There might be a tube in the gripper of robot 1 Figure 5.1 Tube found in robot gripper during initialization Follow the instructions in the alert. When finished, select Start to continue initialization. Error: Volume Detection (3024) If the TIU gripper contains a tube during initialization, initialization stops and the system alerts you:...
Page 173: Complete Power Outage In The Tubes Lost At Robot 1 During
Troubleshooting General Information Error: Tube expected in robot gripper cannot be found If a tube is expected in the robot gripper during initialization but cannot be found, the system prompts you to search for the lost tube in the system. When finished, select Start to continue initialization.
Page 174: Operation,
In the LIS, check the tube status of the tubes you removed. Modify if necessary. In this situation, it is the operator's responsibility to process the tubes according to laboratory procedure. If you cannot run or initialize the system, contact your Beckman Coulter representative. Tubes Lost at Robot 1 During Operation Problem Robot 1 lost a tube.
Page 175: System Freeze
Troubleshooting System Freeze System Freeze Problem The AutoMate PC freezes. The PC needs to be restarted and tubes are still on the system. Cause System malfunction. Remedy Turn off the system using the main power supply switch at the rear of the AutoMate. Ensure that the instrument remains at standstill.
Page 176: Signal Light (Option)
If you cannot run or initialize the system, contact your Beckman Coulter representative. Signal Light (Option) If your system has a signal light, the light color and an acoustic signal alert you to errors.
Page 177: Aliquot Module Troubleshooting
Troubleshooting Aliquot Module Troubleshooting Follow the specific troubleshooting instructions to resolve the issue. Turn on the AutoMate using the switch under the monitor. Wait until initialization is finished. Press Start Aliquot Module Troubleshooting Certain errors cause the aliquot module to stop operating. Keep a vacuum cleaner in the laboratory to clean broken tube debris from the conveyor belts to prevent transport blockages.
Page 178 Printer Error States On the printer display, the diagnostic system indicates any errors and the error type. Report the printer error state to your Beckman Coulter representative. Applicator Error Messages Clear printer errors • Select Cancel to clear the error results.
Page 179: Error: Pump 01 (3005) Tip Is
This error indicates a problem with the liquid level detection (LLD). There can be multiple causes: • Only a clot is detected in the tube, but no liquid. Follow the Remedy steps. • The liquid level detection (LLD) is defective. Contact your Beckman Coulter representative. Remedy...
Page 180: Aspiration Errors - Many Tubes Sent To Error
Troubleshooting Aliquot Module Troubleshooting The pipette tip is lowered to the bottom of the tube. Hold the tube in place with one hand. At the same time, manually move the pipetting unit of robot 3 up. It should remain in the upper position after you release it. Caution Biohazardous material can be spilled or cause contamination! Hold the tube in place to avoid that the tube is pulled up by the pipettor.
Page 181: Led Status Indicator On
Clean the Tip Carrier of the EZ Pipettor Air or foam detected while aspirating. • Replace the Pump Syringe • Contact your Beckman Coulter representative • Visually inspect the sample tubes and confirm if they Tip clogged while aspirating contain clots or fibrin •...
Page 182: Tube Jam At The Tube Hopper
LED signals. The type of error is indicated by the intervals in which the blue LED flashes. If the red LED does not turn off after aliquoting begins or it lights during operation, contact your Beckman Coulter representative. Important The error cannot be resolved by component initialization.
Page 183: Tube Jam Inside The Tube
Troubleshooting Aliquot Module Troubleshooting Cause The top tube cannot drop through the hopper because the previous tube blocks the path. Remedy Access the back of the aliquot module. Push the top tube upwards and hold it. Move the TTU towards the tube lift get-position so that you can reach the tube. Remove both tubes.
Page 184: Tube Jam At The Tube Hopper
Troubleshooting Aliquot Module Troubleshooting Remedy Access the back of the aliquot module. Manually clear tubes from the TTU to clear the path through the hopper. Access the front of the aliquot module. Pull out the tube drawer completely. Insert a pen or similar object into the tube hopper from the right side. Push against the tubes until the hopper is completely empty.
Page 185 Troubleshooting Aliquot Module Troubleshooting Figure 5.6 Top view of the tube hopper with the slider assembly in its default position Pull out the slider completely. Tubes start falling out. Empty the tubes. If the tube jam is cleared, skip step 7 to step 15. Continue with step If tubes are still jammed, depressurize the compressor.
Page 186 Troubleshooting Aliquot Module Troubleshooting Turn the switch from ‘I’ to ‘0’ to switch off the compressor. Figure 5.7 Jun Air Quiet Air 6-4 5-16 B67471B...
Page 187 Troubleshooting Aliquot Module Troubleshooting Figure 5.8 Jun Air 87R-4P Turn the compressor vent valve counterclockwise to depressurize the compressor. Figure 5.9 Jun Air Quiet Air 6-4 5-17 B67471B...
Page 188 Troubleshooting Aliquot Module Troubleshooting Figure 5.10 Jun Air 87R-4P Wait until the compressor is depressurized. The compressor stops releasing air. The jammed tube should now be released. Remove the jammed tube. Replace the slider in the slotted guidance. Close the vent valve. Confirm that the ring valve is closed. Turn the switch from '0' to 'I'.
Page 189: Tube Feeding
Troubleshooting Aliquot Module Troubleshooting Initialize the aliquot module. Select Components> Aliquot module> Initialize. Initialize the printer. Select Components> Aliquot module> Initialize printer. Tube Feeding Interrupted Problem Tube feeding is interrupted. Cause When the tube drawer is closed too hard, single tubes can get stuck in the container mechanics.
Page 190: Tube Fragments In The Ttu
Troubleshooting Aliquot Module Troubleshooting Remedy Remove all tubes between the top feed plate and glider plate. Return the container to its working position. Caution Carefully close the drawer to avoid breaking tubes. The container should appear as shown here. Figure 5.13 Top feed plate, correct position Tube Fragments in the TTU Module Problem...
Page 191: Tube Present Sensor
Troubleshooting Aliquot Module Troubleshooting Figure 5.14 Tube Transfer Unit (TTU) If you find a tube in the TTU, remove it manually. Thoroughly clean the area with a vacuum cleaner. Initialize the aliquot module. Select Components> Aliquot module> Initialize. Tube Present Sensor Problems For maximum safety when processing secondary tubes, two sensors verify the height position and placement of secondary tubes on conveyor belt 2.
Page 192: Troubleshooting Microtiter Plates
LIS. The LIS confirms the position and number of all MTPs when a drawer is closed, so that several error conditions can occur. We cannot provide a list of all possible error messages. System alerts provide instructions on actions to take. Contact your Beckman Coulter representative for support. MTP Not Accepted Problem...
Page 193: Troubleshooting The No Order Buffer
Troubleshooting Troubleshooting The No Order Buffer Rack Cause The LIS does not recognize the MTPs. The LIS has a batch list of all MTP numbers that are available for the laboratory. Remedy Use MTPs that are known to the LIS. Duplicate Plate Error Problem The same MTPs are used on different systems.
Page 194: No Order Buffer Rack Frequently
If the problem is not resolved, contact your local Beckman Coulter representative for support. Camera Troubleshooting Check Tube Pictures If a Beckman Coulter representative asks you to inspect the camera, follow these steps. Select Camera at the bottom of the screen to open the camera inspection window. 5-24 B67471B...
Page 195 The inspection window has to be kept minimized during operation. To prevent interface errors, do not close or exit this window. Continue with any additional steps requested by your Beckman Coulter representative. Error: The Cap Color ID "-2" could not be found in the file tubes.ini...
Page 196 Troubleshooting Camera Troubleshooting 5-26 B67471B...
Page 197 APPENDIX A AutoMate 2500 Family Documents Related Documents Your Beckman Coulter documentation can be found on our website at www.beckmancoulter.com. • AutoMate 2500 Family Instructions for Use • AutoMate 2500 Family Reference Manual • ISB Instructions for Use • Sorting-Drive Client Instructions for Use •...
Page 198 AutoMate 2500 Family Documents Related Documents B67471B...
Page 199 AutoMate 2500 Family — Beckman Coulter laboratory automation product line for sorting and aliquoting samples. LIS — Laboratory Information System. Primary tube — The tube that arrives from the customer.
Page 200 Glossary Glossary-2 B67471B...
Page 201 Index Buttons Initialize ~ Start ~ Stop ~ Errors Exchange Parafilm Gripper remove tube 2-29 Parafilm exchange Recapper exchange the film cassette Software start 2-11 System switch on 2-10 Index-1 B67471B...
Page 202 Index Index-2 B67471B...

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Beckman Coulter AutoMate 2500 Series Instructions For Use Manual

CHAPTER 1 System Overview

CHAPTER 2 Run the System

CHAPTER 3 User Maintenance

System no

CHAPTER 4 Recapper Operation and Maintenance

CHAPTER 5: Troubleshooting

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Troubleshooting

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