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Summary of Contents for Beckman Coulter JS-4.750
- Page 1 Instructions For Use JS-4.750 Swinging-Bucket Rotor For Use in the Beckman Coulter Avanti J-15 Series Centrifuges B80289AF August 2020 Beckman Coulter, Inc. 250 S. Kraemer Blvd. Brea, CA 92821 U.S.A.
- Page 2 B80289AF (August 2020) © 2020 Beckman Coulter, Inc. All rights reserved. Beckman Coulter, the stylized logo, and the Beckman Coulter product and service marks mentioned herein are trademarks or registered trademarks of Beckman Coulter, Inc. in the United States and other countries.
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Page 3: Revision Status
Revision Status Initial Issue B80289AA, 05/2017 Issue AB, 08/2017 Changes or additions were made to: Specifications; Loading the Buckets; Using Blood Bag Cups; Symmetrical and Balanced Loading. Issue AC, 02/2018 Changes or additions were made to: Aerosolve Cannisters, footnote * on page -5; Multi-well Plate Carriers;... - Page 4 Revision Status B80289AF...
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Page 5: Safety Notice
Contact Beckman Coulter, Inc. urges its customers and employees to comply with all national health and safety standards such as the use of barrier protection. This may include, but is not limited to, protective eyewear, gloves, and suitable laboratory attire when operating or maintaining this or any other automated laboratory instrumentation. -
Page 6: Js-4.750 Rotor Safety Precautions
Beckman Coulter. Use of other manufacturers’ components or accessories in the JS-4.750 rotor may void the rotor warranty and should be prohibited by your laboratory safety officer. Only the components and accessories listed in this publication should be used in this rotor. -
Page 7: Radiation Safety
It is your responsibility to decontaminate components of the instrument before requesting service by a Beckman Coulter Representative or returning parts to Beckman Coulter for repair. Beckman Coulter will NOT accept any items which have not been decontaminated where it is appropriate to do so. - Page 8 Safety Notice Chemical and Biological Safety WARNING Risk of personal injury or equipment damage. Ethanol is a flammability hazard. Do not use it in or near operating centrifuges. WARNING Risk of personal injury or property damage. The rotor and accessories are not designed for use with materials capable of developing flammable or explosive vapors.
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Page 9: Mechanical Safety
Beckman Coulter offers the Field Rotor Inspection Program (FRIP). This program involves a visit to your laboratory by a specially trained Beckman Coulter representative, who will inspect all of your... -
Page 10: California Prop 65 Warning
It is your responsibility to decontaminate components of the instrument before requesting service by a Beckman Coulter Representative or returning parts to Beckman Coulter. Beckman Coulter will NOT accept any items which have not been decontaminated where it is appropriate to do so. If any parts are returned, they must be enclosed in a sealed plastic bag stating that the contents are safe to handle and are not contaminated. -
Page 11: Js-4.750 Swinging-Bucket Rotor Safety Messages
Safety Notice JS-4.750 Swinging-Bucket Rotor Safety Messages JS-4.750 Swinging-Bucket Rotor Safety Messages CAUTION Risk of equipment damage. The drive shaft can be damaged if the rotor is forced sideways or dropped onto it. Never force or drop the rotor yoke onto the centrifuge drive shaft. - Page 12 Safety Notice JS-4.750 Swinging-Bucket Rotor Safety Messages CAUTION Risk of equipment damage. Corrosion begins in scratches and may open fissures in the rotor with continued use. Do not use sharp tools on the rotor that could cause scratches in the rotor surface.
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Page 13: Table Of Contents
Chemical and Biological Safety, vii Mechanical Safety, ix California Prop 65 Warning, x Cleaning, x Maintenance, x Disposal, x JS-4.750 Swinging-Bucket Rotor Safety Messages, xi JS-4.750 Swinging-Bucket Rotor, 1 Specifications, 1 Description, 2 Bio-Safety, 3 Serial Number, 3 Accessories, 3... - Page 14 Disinfection, 32 Tube Breakage, 33 Aerosolve Cannisters, 33 SST or Corvac Tubes, 33 Disposal Information, 35 Storage, 35 Troubleshooting, 35 Returning a Rotor, 35 Supply List, 36 Replacement Rotor Parts, 36 Other, 37 Glossary Beckman Coulter, Inc. JS-4.750 Warranty Related Documents...
- Page 15 Illustrations Illustrations Serial number on rotor, 3 Examples of Correctly and Incorrectly Loaded Buckets, 10 Assembling a Modular Disk Adapter, 10 Blood Bag Loading Procedures, 13 Examples of Correctly and Incorrectly Loaded Multi-well Plates, 16 Lubricating Pivot Pin/Bucket Contact Areas, 29...
- Page 16 Tables Tables Adapters and Blood Bag Cups Available for the JS-4.750 Rotor, 20 Beckman Coulter Tubes and Bottles for the JS-4.750 Rotor, 22 Beckman Coulter Labware for Use with Multi-well Plate Carriers, 25 Commercially Available Labware for Use with Multi-well Plate...
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Page 17: Js-4.750 Swinging-Bucket Rotor
JS-4.750 Swinging-Bucket Rotor Specifications Maximum Speed using tube-and-bottle buckets Avanti J-15 ................4550 RPM Avanti J-15R (120 VAC) ............4550 RPM Avanti J-15R (200-230 VAC) ..........4750 RPM using multi-well plate carriers Avanti J-15 ................4350 RPM Avanti J-15R................4450 RPM Critical speed range ............1400 - 1600 RPM... -
Page 18: Description
4. Carrier 5. Carriage This Beckman Coulter rotor has been manufactured in an ISO 9001 or 13485 facility for use with the Beckman Coulter Avanti J-15 Series centrifuges. The JS-4.750 is a four-place swinging-bucket rotor. The rotor buckets carry adapters that allow centrifugation of a wide range of tube and bottle sizes (from 1.5 mL reaction vials to 750 mL bottles) -
Page 19: Bio-Safety
The covers, which are held in place by attached latch assemblies, can be used with some bottles (see Table 2, Beckman Coulter Tubes and Bottles for the JS-4.750 Rotor), and all blood bags, cell culture flasks, and tubes listed in this manual. The covers will contain liquids and broken tube particles, reducing the need to clean the centrifuge chamber and allowing the user to take appropriate precautions before opening the covers in the event of tube breakage. -
Page 20: Bottle Adapters
JS-4.750 Swinging-Bucket Rotor Description Tubes are supported in modular disk adapters, which can also serve as tube racks in the laboratory. The adapter disks are color coded by the tube size they accommodate (see Table 1); the number of disks used in an adapter assembly depends upon the length of tubes used. A tube decanter is available to hold either 10 mm or 12 mm tubes securely in the blue adapter, allowing all the tubes to be decanted at once. -
Page 21: Aerosolve Cannisters
Beckman Coulter centrifuge, when used and maintained as instructed. Aerosolve cannisters hold a variety of tube sizes in racks, or they can be used as 500 mL wide-mouth bottles. -
Page 22: Multi-Well Plate Carriers
Refer to publication GX-TB-012 for use and care of covers. Run Preparation Specific information about the JS-4.750 rotor is given here. Information about the use and care of the centrifuge is contained in the centrifuge manual, which should be used together with this manual for complete rotor and centrifuge operation. -
Page 23: Prerun Safety Checks
Check the chemical compatibilities of all materials used (refer to Chemical Resistances, publication IN-175). Verify that the tubes, bottles, and accessories being used are listed in Table 1, Adapters and Blood Bag Cups Available for the JS-4.750 Rotor. • Table 2, Beckman Coulter Tubes and Bottles for the JS-4.750 Rotor. -
Page 24: Loading The Buckets
Symmetrical and Balanced Loading Beckman Coulter supplies buckets and carriers in weight-matched sets to make balancing easier (the weight and manufacturing date are marked on each bucket and carrier). Modular disk adapters are also sold in weight-matched sets. -
Page 25: Using Tube And Bottle Buckets
JS-4.750 Swinging-Bucket Rotor Run Preparation It is not necessary to completely fill all tubes or positions in buckets; however, partially filled buckets must be balanced with respect to the bucket pivotal axis (see Figure 2). In multi-tube adapters, each tube should be placed so that its weight is balanced by a tube in a diametrically opposite position across the pivotal axis in the same adapter. -
Page 26: Using Tubes
JS-4.750 Swinging-Bucket Rotor Run Preparation Figure 2 Examples of Correctly and Incorrectly Loaded Buckets 1. Balanced Load (Correct) 3. Unbalanced Load (Incorrect) 2. Pivotal Axes of Buckets 4. Center of Rotation NOTE Contents of opposing buckets must be the same and each bucket must be balanced on its pivotal axis. - Page 27 JS-4.750 Swinging-Bucket Rotor Run Preparation Position one of the disks so that its grooves are aligned with the bail. • Push the disk down until the bail snaps into the grooves. Add more disks in this way until the height of the assembly is nearly as tall as the tubes you will be using.
- Page 28 Aerosolve cannisters can be used as wide-mouth bottles or with tubes in racks that are specially designed to fit in the cannisters. Table 1, Adapters and Blood Bag Cups Available for the JS-4.750 Rotor lists the Aerosolve tube racks and the number and sizes of tubes they accommodate. Pads (361269) must be placed beneath the cannisters in the buckets to provide support and prevent the cannisters from being damaged during centrifugation.
- Page 29 JS-4.750 Swinging-Bucket Rotor Run Preparation Sandwich the tubing between the blood bag and any transfer packs (see Figure Figure 4 Blood Bag Loading Procedures 1. Blood Bag Label 4. Blood Bag Seam 2. Blood Bag Seam Aligned with 5. Blood Bag Cup Pivotal Axis of Bucket 6.
- Page 30 JS-4.750 Swinging-Bucket Rotor Run Preparation Place bucket covers on rotor buckets to prevent blood bags or tubing from contacting the rotor yoke during centrifugation. If only two filled cups are run, place them in opposing buckets. The other two buckets should contain similar “blank”...
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Page 31: Loading Buckets Onto The Yoke
Symmetrical and Balanced Loading NOTE Beckman Coulter supplies carriers in weight-matched sets to make balancing easier (the weight and retirement date are marked on the side of each). To prevent accidental imbalance it is important to keep matched sets of carriers together. -
Page 32: Examples Of Correctly And Incorrectly Loaded Multi-Well Plates
JS-4.750 Swinging-Bucket Rotor Run Preparation Figure 5 Examples of Correctly and Incorrectly Loaded Multi-well Plates 1. Balanced Load (Correct) 4. Center of Rotation 2. Unbalanced Load (Incorrect) 5. Examples of Symmetrically Loaded Multi-well Plates (load opposite plates the same way) 3. - Page 33 NOTE Up to three Beckman Coulter plates, separated by cap strips, can be run per carrier at maximum multi-well plate speeds. If running four Beckman Coulter plates, cap strips cannot be used due to height limitations; reduce run speed to 2700 RPM. Observe manufacturer’s run speed and stacking limitations if using other manufacturers’...
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Page 34: Operation
JS-4.750 Swinging-Bucket Rotor Operation CAUTION Risk of equipment damage. Never run the rotor with only two positions filled. All four positions on the rotor must contain buckets or carriers during a run. If two tube-and-bottle buckets and two multi-well plate carriers are run, position the similar buckets opposite each other. -
Page 35: Tubes, Bottles, And Accessories
Use with Multi-well Plate Carriers. Beckman Coulter does not recommend the use of one tube in preference to another nor guarantee the acceptability of the sample tube to produce quality results. If you need information on a tube not listed here,... -
Page 36: Labware
Table 3, Beckman Coulter Labware for Use with Multi-well Plate Carriers for Beckman Coulter labware that can be centrifuged in multi- well plate carriers. Table 1 Adapters and Blood Bag Cups Available for the JS-4.750 Rotor MODULAR DISK ADAPTERS (polypropylene) Color Nom. - Page 37 JS-4.750 Swinging-Bucket Rotor Tubes, Bottles, and Accessories a. Do not autoclave this adapter. Use cold/chemical sterilization techniques only. Refer to Sterilization and Disinfection. CELL CULTURE FLASK ADAPTERS (EPDM) Color Code Number Flasks at Flask RCF at Maximum Part Number Flask Size (cm per Adapter Speed (´...
- Page 38 Tube racks used with Aerosolve cannisters do not provide full tube support; some manufacturers’ plastic and glass tubes cannot withstand the maximum forces generated by this rotor when used in these racks. Beckman Coulter highly recommends that you pretest other manufacturers’...
- Page 39 JS-4.750 Swinging-Bucket Rotor Tubes, Bottles, and Accessories Table 2 Beckman Coulter Tubes and Bottles for the JS-4.750 Rotor (Continued) OPEN-TOP TUBES Dimensions Volume Description Part Number Adapter (mL) Set of Two Set of Four 18 ´ 98 mm polypropylene 342082...
- Page 40 JS-4.750 Swinging-Bucket Rotor Tubes, Bottles, and Accessories CONICAL TUBES Dimensions Volume Description Part Number Adapter (mL) Set of Two Set of Four 62 ´ 141 mm polycarbonate (with cap) 356987 349946 (each) 356983 (PKG 6) 356985 62 ´ 141 mm...
- Page 41 Replacement bottle cap part number is 356263 (set of 6). e. Do not load the 750 mL bottle directly into the bucket; always use the adapter. Table 3 Beckman Coulter Labware for Use with Multi-well Plate Carriers Description Volume...
- Page 42 JS-4.750 Swinging-Bucket Rotor Tubes, Bottles, and Accessories Table 3 Beckman Coulter Labware for Use with Multi-well Plate Carriers Description Volume Part Number Available Accessory Description Part Number Deep-well polypropylene plate, 1 mL/well 267006 267002 (pkg/10) Cap strip, non-sterile 96-well, non-sterile...
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Page 43: Run Speeds
Up to three Beckman Coulter plates can be run per carrier at maximum multi-well plate speeds. If running four Beckman Coulter plates, reduce run speed to 2700 RPM. Observe manufacturer’s run speed and stacking limitations if using other manufacturers’... -
Page 44: Care And Maintenance
JS-4.750 Swinging-Bucket Rotor Care and Maintenance Care and Maintenance Maintenance CAUTION Risk of equipment damage. Corrosion begins in scratches and may open fissures in the rotor with continued use. Do not use sharp tools on the rotor that could cause scratches in the rotor surface. -
Page 45: Lubricating Pivot Pin/Bucket Contact
JS-4.750 Swinging-Bucket Rotor Care and Maintenance Approximately every 400 runs, and after cleaning and/or autoclaving, wipe the rotor pins and pin sockets (see Figure 6) with a paper towel, then coat the sockets with Paint On Graphite Lubricant (977212). • Allow the lubricant to dry for at least 5 minutes before installing the rotor in a centrifuge. -
Page 46: Cleaning
JS-4.750 Swinging-Bucket Rotor Care and Maintenance Cleaning CAUTION Risk of equipment damage. Salts and other corrosive materials can damage the rotor and rotor components. Wash the rotor and rotor components immediately if salts or other corrosive materials are used or if spillage has occurred, according to your laboratory safety procedures. -
Page 47: Cell Culture Flask Adapters
If aluminum rotor components become contaminated with radioactive material, decontaminate them using a solution that will not damage the anodized surfaces. Beckman Coulter has tested a number of solutions and found two that do not harm anodized aluminum: RadCon Surface Spray or †... -
Page 48: Sterilization And Disinfection
JS-4.750 Swinging-Bucket Rotor Care and Maintenance While Beckman Coulter has tested these methods and found that they do not damage the rotor or components, no guarantee of decontamination is expressed or implied. Consult your laboratory safety officer regarding the proper decontamination methods to use. -
Page 49: Tube Breakage
JS-4.750 Swinging-Bucket Rotor Care and Maintenance While Beckman Coulter has tested these methods and found that they do not damage the rotor or components, no guarantee of sterility or disinfection is expressed or implied. When sterilization or disinfection is a concern, consult your laboratory safety officer regarding proper methods to use. - Page 50 JS-4.750 Swinging-Bucket Rotor Care and Maintenance Decant any remaining water and dislodge the tube adapter (do not remove it) over a waste container. WARNING Risk of personal injury or property damage. The rotor and accessories are not designed for use with materials capable of developing flammable or explosive vapors.
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Page 51: Disposal Information
JS-4.750 Swinging-Bucket Rotor Troubleshooting Rinse with water and air dry the cannister and tube rack. Disposal Information Clean and decontaminate the rotor per the Care and Maintenance section of this manual before disposal. Users are encouraged to check with local waste disposal authorities for specific disposal requirements. -
Page 52: Supply List
Contact us for detailed information on ordering parts and supplies. For your convenience, a partial list is given below. Replacement Rotor Parts JS-4.750 rotor assembly (tube-and-bottle buckets) .... B77580 Tube-and-bottle bucket (set of 2)........392804 Tube-and-bottle bucket cover (set of 2)...... -
Page 53: Other
JS-4.750 Swinging-Bucket Rotor Supply List Other NOTE For MSDS information, go to the Beckman Coulter website at www.beckman.com. Adapters ..................Table 1 Tubes and bottles............... Table 2 Multi-well Plate labware Table 3 Soft rubber roller (for aluminum foil lids) 538618 Tube decanter (for use with blue adapter)........ - Page 54 JS-4.750 Swinging-Bucket Rotor Supply List 1-38 B80289AF...
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Page 55: Glossary
As the k factor decreases, rotor efficiency effective and safe for Beckman Coulter rotors increases. and accessories. 2.53 10 ´... - Page 56 Glossary UI — User Interface Yoke — The stainless steel structure that mounts on the centrifuge drive shaft and hold the buckets or carriers. Glossary-2 B80289AF...
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Page 57: Beckman Coulter, Inc
Subject to the conditions specified below and the warranty clause of the Beckman Coulter, Inc., terms and conditions in effect at the time of sale, Beckman Coulter agrees to correct either by repair or, at its election, by replacement, any defects of material or workmanship which develop within seven (7) years after delivery... - Page 58 Beckman Coulter, Inc. JS-4.750 Warranty Warranty-2 B80289AF...
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Page 60: Related Documents
PN B80290 Instructions for Using the Anti-Rotation Anchoring Kit to Secure the Avanti J-15 Series Benchtop Centrifuges PN B80291 JS-4.750 Swinging-Bucket Rotor and JA-10.100 Fixed Angle Rotor Safety Manual PN C01058 Avanti J-15 Series Centrifuge Quick Start Guide PN C01864...
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