Beckman Coulter Microfuge 22R Instruction Manual
  1. Manuals
  2. Brands
  3. Beckman Coulter Manuals
  4. Laboratory Equipment
  5. Microfuge 22R
  6. Instruction manual
Beckman Coulter Microfuge 22R Instruction Manual

Beckman Coulter Microfuge 22R Instruction Manual

Centrifuge
Hide thumbs
Table of Contents

Advertisement

Quick Links

Download this manual
MMR-IM-5A

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the Microfuge 22R and is the answer not in the manual?

Questions and answers

Subscribe to Our Youtube Channel

Related Manuals for Beckman Coulter Microfuge 22R

Summary of Contents for Beckman Coulter Microfuge 22R

  • Page 1 MMR-IM-5A...
  • Page 2 Symbol Simbolo Symbol Title Titel Titre Titulo Titolo Symbole Símbolo Dangerous voltage Gefährliche elektrische Spannung Courant haute tension Voltaje peligroso Pericolo: alta tensione Attention, consult accompanying documents Achtung! Begleitpapiere beachten! Attention, consulter les documents joints Atención, consulte los documentos adjuntos Attenzione: consultare le informazioni allegate On (power) Ein (Netzverbindung)

  • Page 3: Safety Notice

    SAFETY NOTICE This safety notice summarizes information basic to the safe operation of the equipment described in this manual. The international symbol displayed above is a reminder that all safety instructions should be read and understood before installation, operation, mainte- nance, or repair of this centrifuge.
  • Page 4 • Use only the rotors and accessories designed for use in this centrifuge. • Before starting the centrifuge, make sure that the rotor lid is properly installed and the tie- down screw is securely fastened. • Do not exceed the maximum rated speed of the rotor in use. •...
  • Page 5 It is your responsibility to decontaminate the centrifuge and accessories before requesting service by a Beckman Coulter representative. RoHS NoticE These labels and materials declaration table (the Table of Hazardous Substance’s Name and Concentration) are to meet People’s Republic of China Electronic Industry Standard SJ/T11364-2006 “Marking for Control of Pollution Caused by Electronic Information Pro-...
  • Page 7 MMR-IM-5AC November 2013 Microfuge 22R Centrifuge Instruction Manual © 2013 Beckman Coulter, Inc.
  • Page 9 Contents Page INTRODUCTION Certification ..........Scope of Manual.

  • Page 10: Table Of Contents

    Table des matières Page Controls and Indicators ........1-6 Power Switch.
  • Page 11 Page Figure 1-1. The Microfuge 22R Centrifuge ......1-2 Figure 1-2.
  • Page 12 Table des matières...

  • Page 13: Scope Of Manual

    Introduction CERTIFICATION To ensure full system quality, Beckman Coulter Microfuge 22R cen- trifuges are manufactured in an ISO 9001 or 13485 facility. They have been designed and tested to be compliant (when used with Beck- man Coulter rotors) with the laboratory equipment requirements of applicable regulatory agencies.

  • Page 14: Notes, Cautions, And Warnings

    Introduction NOTE If the centrifuge is used in a manner other than specified in this manual, the safety and perfor-mance of this equipment could be impaired. Further, the use of any equipment other than that recommended by Beckman Coulter has not been evaluated for safety. Use of any equipment not specifically recommended in this manual and/or the applicable rotor manual is the sole responsibility of the user.

  • Page 15: Cfc-Free Centrifugation

    CFC-FREE CENTRIFUGATION To ensure minimal environmental impact, no CFCs are used in the manufacture or operation of Microfuge 22R centrifuges. RADIO INTERFERENCE This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to Part 15 of FCC Rules. These...

  • Page 16: Canadian Regulations

    For Beckman Coulter products bearing this label please contact your dealer or local Beckman Coulter office for details on the take back program that will facilitate the proper collection, treatment,...

  • Page 17: Centrifuge Function

    CENTRIFUGE FUNCTION, SPECIFICATIONS, AND SAFETY FEATURES CENTRIFUGE FUNCTION CENTRIFUGE FUNCTION The Beckman Coulter Microfuge 22R (Figure 1-1) is a microproces- sor-controlled refrigerated benchtop centrifuge that generates centri- fugal forces required for a wide variety of applica-tions. Together with the Beckman Coulter rotors, designed specifically for use in this centrifuge, applications include: •...

  • Page 18: Figure 1-1. The Microfuge 22R Centrifuge

    Description Figure 1-1. The Microfuge 22R Centrifuge SPÉCIFICATIONS Only values with tolerances or limits are guaranteed data. Values without tolerances are informative data, without guarantee. Set speed ........500 to 14 000 rpm Set time .

  • Page 19: Available Rotors

    Description AVAILABLE ROTORS Refer to the applicable rotor manuals for complete rotor descriptions. Max. Rotor Manual Rotor Profile and Description (× g) Capacity Number F301.5 Fixed Angle (45° Angle) = 99.5 mm 14,000 21,920 30 × 2.2 mL MMR-TB-002 F241.5P Fixed Angle (45° Angle) = 82.5 mm 14,000 17,970...

  • Page 20: Safety Features

    Description SAFETY FEATURES Microfuge 22R centrifuges have been designed and tested to operate safely indoors at altitudes up to 2 000 m (6 562 ft). Instrument safety features include: • The door has an electromechanical door-locking mechanism to prevent operator contact with spinning rotors. When the door is closed it locks automatically.

  • Page 21: Rotor Chamber

    Description CHASSIS HOUSING The housing is a sheet-steel casing. The control panel is covered by a protective overlay made of coated polycarbonate. DOOR The sheet-steel door has a strobe port for speed verification. A gasket system around the chamber opening assures sealing. (Centrifuge gas- kets have not been designed as bioseals for aerosol containment.) An electromechanical door lock system keeps the door locked when a run is in progress and can be opened only when the rotor is stopped.

  • Page 22: Temperature Sensing And Control

    Description TEMPERATURE SENSING AND CONTROL With the power on, the temperature control system is activated when the door is closed. The run temperature can be set between –10 and +40°C. If no set temperature is entered, the centrifuge automatically selects the last entered temperature. A thermistor in the rotor chamber continuously monitors chamber temperature.

  • Page 23: Figure 1-2. The Control Panel

    Description °C short DOOR Imbalance x1000 START ∞ x1000 14 18 14 18 STOP 4 6 8 10 14 18 22 time rpm/rcf – temp. Figure 1-2. The Control Panel Control Knobs Run parameters are set with the control knobs. The left-hand knob is used to select the parameter (time, speed, or temperature), then values are set using the right-hand knob.
  • Page 24 Description • A continuous (hold) run can be selected by turning the timer to 8. Lighted bars count elapsed run time in 1-minute increments, and “min” is displayed. After 30 minutes the timer will stop showing elapsed time, but the run will continue until the STOP key is pressed.
  • Page 25 Description °C short x1000 ∞ x1000 10 14 18 6 8 10 14 18 22 If the temperature is reset below the existing temperature while a run is in progress, the new set temperature is indicated by a flashing bar. °C short x1000...
  • Page 26 Description If the speed is set below the existing run speed while a run is in progress, the new set speed is indicated by a flashing bar and the drive decelerates to the new value. °C short x1000 ∞ x1000 10 14 18 6 8 10 14 18 22 Door...

  • Page 27: Installation

    Installation INSTALLING THE CENTRIFUGE WARNING This centrifuge weighs 41.0 kg (90.4 lb). DO NOT attempt to lift or move it without assistance from another person. WARNING Do not place the centrifuge near areas containing flammable reagents or combustible fluids. Vapors from these materials could enter the centrifuge’s air system and be ignited by the motor.

  • Page 28: Figure 2-1. Dimensions Of The Microfuge 22R Centrifuge

    30.0 23.0 58.5 14.4 36.5 Figure 2-1. Dimensions of the Microfuge 22R Centrifuge • Ambient temperatures during operation should not be lower than 4°C (39°F) or higher than 35°C (95°F). Relative humidity should not exceed 80% (noncondensing). NOTE During transport between areas with varying temperatures, condensation may occur inside the centrifuge.

  • Page 29: Transportation Safety Devices

    Installation TRANSPORTATION SAFETY DEVICES The centrifuge has a shipping screw on the bottom plate and a plastic foam stabilizer installed in the rotor chamber to prevent damage to the drive motor during transit. When installation of the centrifuge is complete: •...

  • Page 30: Test Run

    Installation TEST RUN We recommend that you make a test run to ensure that the centrifuge is in proper operating condition following shipment. See Section 3 for instructions on operating the centrifuge. After completing the test run, return the preaddressed warranty card included with this literature.

  • Page 31: Operation

    Operation WARNING Handle infectious samples according to good laboratory procedures and methods to prevent spread of disease. Because spills, operator error, or tube failure may gene- rate aerosols, observe proper safety precautions for aerosol containment. Do not run toxic, pathogenic, or radioactive materials in this centrifuge without taking appropriate safety precautions.
  • Page 32 Operation Action Result 1. Press the power switch to on Power is applied to the centrifuge; all LEDs on the control panel light (I). up momentarily. 2. Press DOOR and lift the The door will remain open. door up. CAUTION Do not drop the rotor onto the drive shaft.
  • Page 33 Operation Action Result 4. Secure the rotor to the drive shaft with the tie-down screw by holding the rotor with one hand while turning the T- handle rotor wrench to the right (clockwise). NOTE If the rotor is left in the centrifuge between runs, make sure it is seated on the drive shaft and the tie-down screw is tight before each run.

  • Page 34: Starting A Timed Or Hold Run

    Operation STARTING A TIMED OR HOLD RUN Action Result 1. Turn the parameter selection °C knob to rpm/rcf, then use the short +/- knob to select a speed (up x1000 to 14,000 rpm). ∞ x1000 time rpm/rcf 10 14 18 temp.
  • Page 35 Operation Action Result 4. Check that the door is shut and securely latched. 5. Press START. • Timed run—Lighted bars count down time remaining, in 1-minute increments, and “min” is displayed. Lighted bars on the speed scale indicate rotor speed (rpm × 1000 on upper scale, rcf × 1000 on lower scale).

  • Page 36: Starting A Pulse Run

    Operation Action Result A timed run ends automatically when the set time counts down to zero. You can end a hold or timed run in progress for any reason by pressing the STOP key. 6. After the rotor stops spinning The door latch is released.

  • Page 37: Pulse Mode 2

    Operation Action Result 3. Press and hold the START The centrifuge accelerates to the maximum speed and continues as key. long as the key is pressed, decelerating when the key is released. Lighted bars count elapsed run time in 1-second increments, and “sec”...

  • Page 38: Unloading

    Operation Action Result 4. Press adn release the START The centrifuge accelerates to the maximum speed and continues as key. long as the key is pressed, decelerating when the key is released. Lighted bars count elapsed run time in 1-second increments, and “sec”...

  • Page 39: Troubleshooting

    Perform the recommended corrective action in sequence, as listed. If you are unable to correct the problem, call your local Beckman Coulter repre- sentative. To help diagnose and correct the problem, note as much information about the situation as possible: •...

  • Page 40: Error Messages

    Refer to Table 4-2 to determine the nature of the problem and recommended actions. If you are unable to correct the problem, call your Beckman Coulter Field Service repre- sentative. Ceci indique l’erreur n 3...

  • Page 41: Accessing The Rotor In Case Of Power Failure

    Troubleshooting Table 3-2. Error Message Chart. If the recommended action does not correct the problem, call Beckman Coulter Field Service. Numéro d’erreur Description Mesures recommandées Bad tachometer reading Mettre l’instrument à l’arrêt (O) et attendre que le rotor soit à...
  • Page 42 Troubleshooting WARNING The following procedure may expose the ope- rator to the possibility of contact with a spin- ning rotor. Turn the power off (O) and disconnect the centrifuge from the main power source before proceeding. Never attempt to override the door interlock system while the rotor is spinning.
  • Page 43 Troubleshooting 7. Open the door and remove the rotor. 8. Reinsert the release devices and tighten the retaining screws.
  • Page 44 Troubleshooting...

  • Page 45: Care And Maintenance

    For maintenance not covered in this manual, contact Beckman Coul- ter Field Service (1-800-742-2345 in the U.S.A.; customers outside the United States should contact their local Beckman Coulter repre- sentative). Refer to the rotor manual and Chemical Resistances (publication IN-175) for instructions on the care of rotors and rotor accessories.

  • Page 46: Maintenance

    Care and Maintenance MAINTENANCE PREVENTIVE MAINTENANCE Perform the following procedures regularly to ensure satisfactory per- formance and long service life of the centrifuge. • Regularly inspect the interior of the rotor chamber for accu- mula-tions of sample or dust. Clean as required (see CLEANING, below), as these accumulations can result in rotor vibrations.
  • Page 47 Care and Maintenance 3. Pull the fuse holder straight Fuses are accessible. out. 4. Pull the fuse straight out of the fuse holder. 5. Carefully insert a new fuse. Press it straight in until it seats in the fuse holder. 6.

  • Page 48: Cleaning

    Care and Maintenance CLEANING Frequent cleaning will ensure proper operation and prolong the life of the centrifuge. Always clean up spills when they occur to prevent cor- rosives or contaminants from drying on component surfaces. NOTE Before using any cleaning methods except those recommended by the manufacturer, users should check with the manufacturer that the proposed method will not damage the equipment.

  • Page 49: Sterilization And Disinfection

    Resistances for more information regarding chemical resistance of centrifuge and accessory materials. While Beckman Coulter has tested these methods and found that they do not damage the centrifuge, no guarantee of sterility or disinfection is expressed or implied. When sterilization or disinfection is a concern, consult your laboratory safety officer regarding proper methods to use.

  • Page 50: Returning A Centrifuge

    Before returning a centrifuge or accessory for any reason, prior per- mission (a Returned Goods Authorization form) must be obtained from Beckman Coulter, Inc. Contact your local Beckman Coulter office to obtain the RGA form and for packaging and shipping ins- tructions.

  • Page 51: Supply List

    800-742-2345 in the United States, or by contac- ting your local Beckman Coulter office. Contact Beckman Coulter Sales (1-800-742-2345 in the United States; worldwide offices are listed at the back of this manual) for detailed information on ordering parts and supplies. For your conve-nience, a partial list is given below.
  • Page 52 Care and Maintenance...
  • Page 53 CONDITIONS below and the warranty clause of the Beckman Coulter terms and conditions in effect at the time of sale, Beckman Coulter Beckman Coulter shall be released from all obligations under agrees to correct either by repair or, at its election, by...
  • Page 54 Beckman Coulter, Inc. • 250 S. Kraemer Blvd. • Brea, California 92821 Sales and Service: 1-800-742-2345 • Internet: www.beckmancoulter.com ©2013 Beckman Coulter, Inc. tous droits réservés...

Table of Contents