Beckman Coulter Allegra 25R Instruction Manual
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Beckman Coulter Allegra 25R Instruction Manual

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BA-IM-5
®
Allegra
25R Centrifuge
®
Instruction Manual

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Summary of Contents for Beckman Coulter Allegra 25R

  • Page 1 BA-IM-5 ® Allegra 25R Centrifuge ® Instruction Manual...
  • Page 2 Symbol Simbolo Symbol Title Titel Titre Titulo Titolo Symbole Símbolo Dangerous voltage Gefährliche elektrische Spannung Courant haute tension Voltaje peligroso Pericolo: alta tensione Attention, consult accompanying documents Achtung! Begleitpapiere beachten! Attention, consulter les documents joints Atención, consulte los documentos adjuntos Attenzione: consultare le informazioni allegate On (power) Ein (Netzverbindung)
  • Page 3 Safety During Installation and/or Maintenance This centrifuge is designed to be installed by a Beckman Coulter Field Service representa- tive. Installation by anyone other than authorized Beckman Coulter personnel invalidates any warranty covering the instrument.
  • Page 4 Laboratory Biosafety Manual) are handled; materials of a higher group require more than one level of protection. • Dispose of all waste solutions according to appropriate environmental health and safety guidelines. It is your responsibility to decontaminate the centrifuge and accessories before requesting service by Beckman Coulter Field Service.
  • Page 5 BA-IM-5 April 2009 Allegra 25R Centrifuge ® Instruction Manual © 2009 Beckman Coulter, Inc.

  • Page 7: Table Of Contents

    Contents Page INTRODUCTION Certification ..........Scope of Manual.
  • Page 8 Contents Page SECTION 3: OPERATION Summary of Operating Procedures ......3-2 Installing the Rotor........3-3 Setting Run Parameters .
  • Page 9 Figure 1-1. The Allegra 25R Centrifuge....... . . 1-2 Figure 1-2.

  • Page 11: Introduction

    SCOPE OF MANUAL This manual is designed to familiarize you with the Beckman Coulter Allegra 25R centrifuge, its functions, specifications, operation, and routine operator care and maintenance. We recommend that you read...

  • Page 12: Conventions

    Further, the use of any equipment other than that recommended by Beckman Coulter has not been evaluated for safety. Use of any equipment not specifically recommended in this manual and/or the appropriate rotor manual is the sole responsibility of the user.

  • Page 13: Typographic Conventions

    ]) appear in capital START ROTOR letters within brackets. • Display names (for example, SPEED or TIME) appear in bold type. CFC-FREE CENTRIFUGATION To ensure minimal environmental impact, no CFCs are used in the manufacture or operation of Allegra 25R centrifuges.

  • Page 14: Radio Interference

    For Beckman Coulter products bearing this label please contact your dealer or local Beckman Coulter office for details on the take back program that will facilitate the proper collection, treatment,...

  • Page 15: Description

    Description CENTRIFUGE FUNCTION AND SAFETY FEATURES CENTRIFUGE FUNCTION The Beckman Coulter Allegra 25R centrifuge (see Figure 1-1) is a refrigerated benchtop centrifuge that generates centrifugal forces required for a wide variety of applications. Together with the Beckman Coulter rotors designed for use in this centrifuge, the centrifuge applications include: •...

  • Page 16: Safety Features

    Description Figure 1-1. The Allegra 25R Centrifuge SAFETY FEATURES The centrifuge has been designed and tested to operate safely indoors at altitudes up to 2 000 m (6 562 ft). Safety features include the following. • An electromechanical door lock system prevents operator contact with spinning rotors and prevents run initiation unless the door is shut and locked.

  • Page 17: Centrifuge Chassis

    Description CENTRIFUGE CHASSIS HOUSING The centrifuge housing is made of sheet steel and is finished with urethane paint. The control panel overlay is made of coated polycarbonate. DOOR The steel door is secured to the housing by solid hinges. When the door is closed, a motorized locking system engages.

  • Page 18: Drive

    Description DRIVE The asynchronous direct-drive motor is brushless for clean, quiet operation. A tie-down screw is used to attach the rotor to the drive shaft. The resilient suspension ensures that loads will not be disturbed by vibration, and prevents damage to the drive shaft if an imbalance occurs during centrifugation.
  • Page 19 Description System Keys Centrifuge operation is controlled through the system keys. Each key, except [ ], has an LED in the upper left corner that lights PULSE to indicate operational readiness. Unlocks the electromechanical door lock. The instrument will not OPEN DOOR accept this command if the rotor is spinning.
  • Page 20 Description Pressed to select the run duration, using the cursor keys. TIME • Timed run—can be set up to 9 hours, 59 minutes (a minutes parameter exceeding 59 is automatically converted into hours). • Continuous run—timer is set for less than 0 or more than 9 hours, 59 minutes (by pressing the down cursor key after the display reaches 0, or the up cursor key after the display reaches 9:59).

  • Page 21: Name Rating Plate

    The name rating plate is affixed to the rear of the centrifuge. Check that the line voltage agrees with the voltage listed on this name rating plate before connecting the centrifuge. Always mention the serial number and the model number shown when corresponding with Beckman Coulter regarding your centrifuge.

  • Page 22: Specifications

    Description SPECIFICATIONS Only values with tolerances or limits are guaranteed data. Values without tolerances are informative data, without guarantee. Speed Set speed ....to 15 000 rpm in 100-rpm increments Speed control.

  • Page 23: Available Rotors

    Description AVAILABLE ROTORS The following Beckman Coulter rotors can be used in the Allegra 25R centrifuge. The rotors are described in individual manuals that accompany each rotor. Max RCF † Rotor Number of Rotor (× g) Tubes × Nominal Selection...

  • Page 25: Preinstallation Requirements

    Preinstallation Requirements The Allegra 25R centrifuge is designed to be installed by Beckman Coulter Field Service. Installation by anyone other than authorized Beckman Coulter personnel invalidates any warranty covering the centrifuge. Preinstallation requirements have been sent prior to ship- ment of your centrifuge. The following information is provided in case the instrument must be relocated.

  • Page 26: Electrical Requirements

    The plug for use in North America is the NEMA 6-20P; a NEMA 6-20R socket (see Figure 2-2) should be available. (Contact Beckman Coulter field service for specific information regarding local requirements.) To preserve this safety feature:...

  • Page 27: Transportation Safety Device

    (368248) installed on the instrument bottom to prevent damage to the drive motor during transit. When installation of the centrifuge is complete, the Beckman Coulter Field Service representative will remove the transport device. Save it to use during future relocation...
  • Page 29 Operation This section contains centrifuge operating procedures. A summary is provided at the start of this section. If you are an experienced user of this centrifuge, you can turn to the summary for a quick review of operating steps. Refer to the applicable rotor manual for instructions on preparing the rotor for centrifugation.

  • Page 30: Operation

    Operation SUMMARY OF OPERATING PROCEDURES For runs at other than room temperature, refrigerate or warm the rotor beforehand for fast equilibration. With the power switch on, press [ ] and open the OPEN DOOR OPEN DOOR chamber door. Install the rotor according to the applicable rotor manual. Always run the rotor with a balanced load.

  • Page 31: Installing The Rotor

    Operation INSTALLING THE ROTOR Prepare the rotor for centrifugation as described in the applicable rotor manual. For fast temperature equilibration, cool or warm the rotor to the required temperature before the run. Recommended precool period is 30 minutes at 2000 rpm for fixed angle rotors or 1000 rpm for swinging bucket rotors, with the temperature set to 0°C.

  • Page 32: Setting Run Speed

    Operation ➠ NOTE If you select the TS-5.1-500 rotor (selection code S-5.1) a submenu will appear. Select BUC5 for buckets or PLA for microplate carriers. Action Result 3. When the selected rotor Rotor number will be shown in the SPEED display. You can set number is displayed, parameters for the run.

  • Page 33: Setting Run Time

    Operation Setting RCF Action Result 1. Press [ The last digit on the SPEED display flashes. SPEED 2. Press [ ] or [ ] until The corresponding rpm will be automatically calculated and the the required RCF appears, centrifuge will run at the calculated speed. You can check the RCF then press [ during the run by pressing [ ] while the centrifuge is running.

  • Page 34: Setting Acceleration And Deceleration Rates

    Operation Action Result 1. Press [ The last digit of the TEMP °C display flashes. TEMP 2. Press [ ] or [ ] until Entered temperature appears on the TEMP °C display. the required temperature appears, then press [ ENTER SETTING ACCELERATION AND DECELERATION RATES You can select one of ten acceleration rates and ten deceleration rates to protect the gradient and sample-to-gradient interface.

  • Page 35: Starting A Run

    Operation Table 3-1. Acceleration and Deceleration Rates (in Minutes:Seconds) to and from Maximum Speed. Times shown are for the TS-5.1-500 rotor, with the rotor fully loaded, to/from maximum rotor speed of 5000 rpm. (Times are approximate; actual times will vary depending on the rotor in use, the rotor load, run speed, and voltage fluctuations.) Accel/Decel Rate Accel...

  • Page 36: Changing Parameters During A Run

    Operation Action Result Result CAUTION 1. Check that all parameters Do not lift or move the centrifuge while the rotor are correct and the door is is spinning. closed. 2. Press [ • The SPEED display indicates rotor speed in rpm. (You can check START the RCF by pressing the [ ] key.)

  • Page 37: Stopping A Run

    Operation • Deceleration rate can be changed until deceleration begins. (The deceleration rate cannot be changed while the rotor is decelerating.) Use the parameter keys as described under SETTING RUN PARAM- ETERS, above. You must press [ ] after you input each parame- ENTER ter change.
  • Page 38 Operation Action Result 2. After the rotor stops The door can be opened. spinning, press [ OPEN DOOR CAUTION 3. Remove the rotor according If disassembly reveals evidence of leakage, you to the rotor manual. should assume that some fluid escaped the rotor. Apply appropriate decontamination procedures to the centrifuge and accessories.

  • Page 39: Troubleshooting

    Refer to Table 4-1 to determine the nature of the problem. If you are unable to correct the problem, call Beckman Coulter Field Service. To help diagnose and correct the problem, provide as much information as possible: •...
  • Page 40 Troubleshooting Table 3-1. Error Messages Error Number Problem Result Recommended Action 1 through 62, Microprocessor or Deceleration to full After rotor comes to a complete stop turn 69 through 77 mechanical error stop; run cannot be the power off, then wait 15 seconds before restarted until error turning it back on to reset.

  • Page 41: Other Possible Problems

    Table 4-2, along with probable causes, listed in the probable order of occurrence, and corrective actions. Perform the recommended corrective action in sequence, as listed. If you are unable to correct the problem, call Beckman Coulter Field Service. Table 3-2. Troubleshooting Chart...

  • Page 42: Retrieving Your Sample In Case Of Power Failure

    Troubleshooting RETRIEVING YOUR SAMPLE IN CASE OF POWER FAILURE If facility power fails only momentarily, the centrifuge will resume operation when power is restored and the rotor will return to set speed. However, if the rotor comes to a complete stop, you will have to restart the run when the power is restored.

  • Page 43: Figure 4-1. Door Latch Override

    Troubleshooting Figure 4-1. Door Latch Override. (A 10-mm square-head socket wrench can be used if the provided override key is unavailable.)

  • Page 45: Care And Maintenance

    This section contains care and maintenance procedures that should be performed regularly. For maintenance not covered in this manual, contact Beckman Coulter Field Service (1-800-742-2345 in the United States; worldwide offices are listed on the inside back cover of this manual) for assistance. User messages and recommended actions...

  • Page 46: Cleaning

    Care and Maintenance CLEANING Frequent cleaning will ensure proper operation and prolong the life of the centrifuge. Always clean up spills when they occur to prevent corrosives or contaminants from drying on component surfaces. (Be careful not to spill liquid on the instrument where electrical or mechanical components could become damaged.) •...

  • Page 47: Sterilization And Disinfection

    Resistances for more information regarding chemical interactions of instrument and accessory materials. While Beckman Coulter has tested these methods and found that they do not damage the instrument, no guarantee of sterility or disinfection is expressed or implied. When sterilization or disinfection is a concern, consult your laboratory safety officer regarding proper methods to use.

  • Page 48: Supply List

    Care and Maintenance SUPPLY LIST Contact Beckman Coulter Sales (1-800-742-2345 in the United States; worldwide offices are listed on the inside back cover of this manual) for information about ordering parts, supplies, and publica- tions. A partial list is given below for your convenience.
  • Page 49 (1) year after delivery of an Allegra 25R its own authorized service personnel, unless such repair in the centrifuge (the product), to the original buyer by Beckman...
  • Page 55 Beckman Coulter, Inc. • 250 S. Kraemer Blvd. • Brea, California 92821 Sales and Service: 1-800-742-2345 • Internet: www.beckmancoulter.com ©2009 Beckman Coulter, Inc. All rights reserved...

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