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Summarized manual for FCS or other point measurements
Figure 184:
212
Display of the FCS curves in the "Correlation" tab of the annotation file
Using detectors with high quantum efficiency
FCS measurements can only be carried out with detectors with high
quantum efficiency, such as APDs. Using cross-correlation between two
detectors, detector after pulsing effects can be suppressed all the way
to the correlation curve. With pulsed excitation, alternatively, FLCS can
be used to calculate detector after-pulses for the individual detection
channels from the raw data.
A stable count rate for FCS analysis is necessary
For a reliable FCS analysis, the count rate has to be stable. Signal
attenuation due to photobleaching may lead to an increasing FCS
correlation amplitude at long lag times.
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