Leica TCS SP8 SMD User Manual page 156

FLIM Data Acquisition
3. Define a file name in the Setup tab (Chapter 16.3.2).
4. Open the Acquisition tab. Via the stack dialog, you define the first and last image of the
stack and the number of planes (Figure 139).
5. Define the duration of the individual FLIM images as described in Chapter 16.3.3. Use
either Duration of each image or Repetitions, because the last option, Acquire until max
___ photons/pixel, does not allow a fixed period of time to be specified.
6. You can define a time series using the time series dialog (Figure 141). The following
inputs are possible:
• Time Interval: defines the duration between the beginning of an FLIM stack and the
• Duration: Here, enter how long the entire time series is to last. The corresponding
• Stacks: Here, you can specify the number of FLIM stacks to be acquired. The
Figure 141:
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next stack. This time span cannot be shorter than the timespan required for an
individual FLIM stack. To specify the smallest possible duration, select Minimize.
number of FLIM stacks is acquired. The entered duration is automatically corrected
so that the last FLIM stack can be completed.
maximum specified number of FLIM images is 1000.
Defining a time series of xyzt FLIM stacks